Journal of Animal Science, Vol 78, Issue 8 2127-2134, Copyright © 2000 by American Society of Animal Science

JOURNAL ARTICLE

Expression of porcine transcription factors and genes related to fatty acid metabolism in different tissues and genetic populations

S. T. Ding, A. P. Schinckel, T. E. Weber and H. J. Mersmann
USDA/ARS Children's Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine, Houston, TX 77030-2600, USA. sding@bcm.tmc.edu

Several transcription factors are involved in regulating lipid metabolism in various tissues of animals. Adipocyte determination and differentiation-dependent factor 1 (ADD1), peroxisome proliferator activated receptor alpha (PPAR alpha), and peroxisome proliferator activated receptor gamma (PPAR gamma) regulate both lipogenesis and fatty acid oxidation. We determined the tissue distribution and genetic difference in mRNA concentrations of these transcription factors in two genetic populations of pigs (Newsham XL-sired Newsham Landrace x Large White Duroc and Duroc-sired US Yorkshire x Duroc-Landrace). We also determined the tissue distribution and genetic difference in the mRNA concentration of fatty acid synthase (FAS) and acyl-CoA oxidase (ACO). Our data showed that ADD1 was highly expressed in adipose tissue and liver and that mRNA concentrations of ADD1 were similar between the two genotypes. The PPAR alpha mRNA concentration was high in adipose tissue and was similar between the two genotypes. In both populations, PPAR gamma mRNA was detected only in adipose tissue. There was no difference between the two genotypes in PPAR gamma mRNA concentration. The ACO mRNA was expressed in adipose tissue, skeletal muscle, and liver with no difference between genotypes. The FAS mRNA concentration in adipose tissue was seven times higher than that in the liver. There was no detectable FAS mRNA in skeletal muscle. These data support the concept that pig adipose tissue has considerable capability for fatty acid oxidation and synthesis. The uniqueness of expression patterns for FAS and ADD1 mRNA further indicates that adipose tissue is significantly involved in fatty acid and triacylglycerol synthesis in pigs.

This article has been cited by other articles:

				C. H. Chen, P. H. Wang, B. H. Liu, H. H. Hsu, H. J. Mersmann, and S. T. Ding Serum Amyloid A Protein Regulates the Expression of Porcine Genes Related to Lipid Metabolism J. Nutr., April 1, 2008; 138(4): 674 - 679. [Abstract] [Full Text] [PDF]

				Y. H. Yu, S. C. Wu, W. T. K. Cheng, H. J. Mersmann, and S. T. Ding Ectopic expression of porcine peroxisome proliferator-activated receptor {delta} regulates adipogenesis in mouse myoblasts J Anim Sci, January 1, 2008; 86(1): 64 - 72. [Abstract] [Full Text] [PDF]

				S. Luci, B. Giemsa, H. Kluge, and K. Eder Clofibrate causes an upregulation of PPAR-{alpha} target genes but does not alter expression of SREBP target genes in liver and adipose tissue of pigs Am J Physiol Regulatory Integrative Comp Physiol, July 1, 2007; 293(1): R70 - R77. [Abstract] [Full Text] [PDF]

				E. Lord, B. D Murphy, J. A Desmarais, S. Ledoux, D. Beaudry, and M.-F. Palin Modulation of peroxisome proliferator-activated receptor {delta} and {gamma} transcripts in swine endometrial tissue during early gestation. Reproduction, May 1, 2006; 131(5): 929 - 942. [Abstract] [Full Text] [PDF]

				R. Wang, C. Cruciani-Guglielmacci, S. Migrenne, C. Magnan, V. E. Cotero, and V. H. Routh Effects of Oleic Acid on Distinct Populations of Neurons in the Hypothalamic Arcuate Nucleus Are Dependent on Extracellular Glucose Levels J Neurophysiol, March 1, 2006; 95(3): 1491 - 1498. [Abstract] [Full Text] [PDF]

				W. G. Bergen and H. J. Mersmann Comparative Aspects of Lipid Metabolism: Impact on Contemporary Research and Use of Animal Models J. Nutr., November 1, 2005; 135(11): 2499 - 2502. [Abstract] [Full Text] [PDF]

				B. H. Liu, C. F. Kuo, Y. C. Wang, and S. T. Ding Effect of docosahexaenoic acid and arachidonic acid on the expression of adipocyte determination and differentiation-dependent factor 1 in differentiating porcine adipocytes J Anim Sci, July 1, 2005; 83(7): 1516 - 1525. [Abstract] [Full Text] [PDF]

				J. M. Hsu, P. H. Wang, B. H. Liu, and S. T. Ding The effect of dietary docosahexaenoic acid on the expression of porcine lipid metabolism-related genes J Anim Sci, March 1, 2004; 82(3): 683 - 689. [Abstract] [Full Text] [PDF]

				S.-T. Ding, A. Lapillonne, W. C. Heird, and H. J. Mersmann Dietary fat has minimal effects on fatty acid metabolism transcript concentrations in pigs J Anim Sci, February 1, 2003; 81(2): 423 - 431. [Abstract] [Full Text] [PDF]

				F. Gondret and B. Lebret Feeding intensity and dietary protein level affect adipocyte cellularity and lipogenic capacity of muscle homogenates in growing pigs, without modification of the expression of sterol regulatory element binding protein J Anim Sci, December 1, 2002; 80(12): 3184 - 3193. [Abstract] [Full Text] [PDF]