| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Journal of Animal Science, Vol 78, Issue 2 477-487, Copyright © 2000 by American Society of Animal Science
JOURNAL ARTICLE |
A. N. Hristov, T. A. McAllister and K. J. Cheng
Agriculture and Agri-Food Canada, Research Centre, Lethbridge, AB Canada.
The effects of supplying increasing ruminal doses of exogenous polysaccharide-degrading enzymes (EPDE) on rumen fermentation and nutrient digestion were studied using eight ruminally cannulated heifers, four of which were also duodenally cannulated, in a replicated Latin square. The heifers were fed a diet of 85.5% rolled barley grain and 14% barley silage (DM basis), and once daily they were given intraruminal doses of 0 (Control), 100, 200, or 400 g of a preparation containing polysaccharide-degrading enzymes. Enzyme treatment decreased ruminal pH (linear, P<.001) and increased ammonia N (quadratic, P<.001) concentration. The ruminally soluble fraction and effective degradability of feed DM in situ were increased (quadratic response, P<.001) by enzyme treatment. Ruminal administration of EPDE increased ruminal fluid carboxymethylcellulase and xylanase activities linearly (P<.001) and beta-glucanase activity quadratically (P<.01), decreased (quadratic response, P<.05) ruminal fluid viscosity, and did not affect (P>.05) ruminal fluid amylase activity. Elevated levels of fibrolytic activities in the rumen resulted in increased (quadratic, P<.001) carboxymethylcellulase, xylanase, and beta-glucanase (P<.01) activities in duodenal digesta. Duodenal amylase activity and reducing sugar concentration were also increased (quadratic responses, P<.001 and P<.05, respectively) by EPDE. Xylanase activity of fecal DM was increased linearly (P<.05) with increasing ruminal EPDE levels. Apparent digestibilities of DM, crude protein, and NDF were not affected by EPDE supplementation. Enzyme treatment did not affect (P>.05) urinary excretion of allantoin and uric acid, or concentrations of glucose and urea in blood.
This article has been cited by other articles:
|
|
 |
|
  L. A. Giraldo, M. L. Tejido, M. J. Ranilla, S. Ramos, and M. D. Carro Influence of direct-fed fibrolytic enzymes on diet digestibility and ruminal activity in sheep fed a grass hay-based diet J Anim Sci, July 1, 2008; 86(7): 1617 - 1623. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. A. Elwakeel, E. C. Titgemeyer, B. J. Johnson, C. K. Armendariz, and J. E. Shirley Fibrolytic Enzymes to Increase the Nutritive Value of Dairy Feedstuffs J Dairy Sci, November 1, 2007; 90(11): 5226 - 5236. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. A. Reddish and L. Kung Jr. The Effect of Feeding a Dry Enzyme Mixture with Fibrolytic Activity on the Performance of Lactating Cows and Digestibility of a Diet for Sheep J Dairy Sci, October 1, 2007; 90(10): 4724 - 4729. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. A. Giraldo, M. L. Tejido, M. J. Ranilla, and M. D. Carro Effects of exogenous cellulase supplementation on microbial growth and ruminal fermentation of a high-forage diet in Rusitec fermenters J Anim Sci, August 1, 2007; 85(8): 1962 - 1970. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. J. Suarez, C. G. Van Reenen, N. Stockhofe, J. Dijkstra, and W. J. J. Gerrits Effect of Roughage Source and Roughage to Concentrate Ratio on Animal Performance and Rumen Development in Veal Calves J Dairy Sci, May 1, 2007; 90(5): 2390 - 2403. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. M. Tricarico, M. D. Abney, M. L. Galyean, J. D. Rivera, K. C. Hanson, K. R. McLeod, and D. L. Harmon Effects of a dietary Aspergillus oryzae extract containing {alpha}-amylase activity on performance and carcass characteristics of finishing beef cattle J Anim Sci, March 1, 2007; 85(3): 802 - 811. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. E. Foley, A. N. Hristov, A. Melgar, J. K. Ropp, R. P. Etter, S. Zaman, C. W. Hunt, K. Huber, and W. J. Price Effect of barley and its amylopectin content on ruminal fermentation and nitrogen utilization in lactating dairy cows. J Dairy Sci, November 1, 2006; 89(11): 4321 - 4335. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. J. Suarez, C. G. Van Reenen, G. Beldman, J. van Delen, J. Dijkstra, and W. J. J. Gerrits Effects of supplementing concentrates differing in carbohydrate composition in veal calf diets: I. Animal performance and rumen fermentation characteristics. J Dairy Sci, November 1, 2006; 89(11): 4365 - 4375. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. M. DeFrain, A. R. Hippen, K. F. Kalscheur, and J. M. Tricarico Effects of Dietary {alpha}-Amylase on Metabolism and Performance of Transition Dairy Cows J Dairy Sci, December 1, 2005; 88(12): 4405 - 4413. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. B. Dean, A. T. Adesogan, N. Krueger, and R. C. Littell Effect of Fibrolytic Enzymes on the Fermentation Characteristics, Aerobic Stability, and Digestibility of Bermudagrass Silage J Dairy Sci, March 1, 2005; 88(3): 994 - 1003. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. N. Hristov, K. L. Grandeen, J. K. Ropp, and M. A. McGuire Effect of Sodium Laurate on Ruminal Fermentation and Utilization of Ruminal Ammonia Nitrogen for Milk Protein Synthesis in Dairy Cows J Dairy Sci, June 1, 2004; 87(6): 1820 - 1831. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. N. Hristov, S. Ahvenjarvi, T. A. McAllister, and P. Huhtanen Composition and digestive tract retention time of ruminal particles with functional specific gravity greater or less than 1.02 J Anim Sci, October 1, 2003; 81(10): 2639 - 2648. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. N. Hristov and J. K. Ropp Effect of Dietary Carbohydrate Composition and Availability on Utilization of Ruminal Ammonia Nitrogen for Milk Protein Synthesis in Dairy Cows J Dairy Sci, July 1, 2003; 86(7): 2416 - 2427. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Colombatto, F. L. Mould, M. K. Bhat, D. P. Morgavi, K. A. Beauchemin, and E. Owen Influence of fibrolytic enzymes on the hydrolysis and fermentation of pure cellulose and xylan by mixed ruminal microorganisms in vitro J Anim Sci, April 1, 2003; 81(4): 1040 - 1050. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. R. Bowman, K. A. Beauchemin, and J. A. Shelford Fibrolytic Enzymes and Parity Effects on Feeding Behavior, Salivation, and Ruminal pH of Lactating Dairy Cows J Dairy Sci, February 1, 2003; 86(2): 565 - 575. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. A. Beauchemin, D. Colombatto, D. P. Morgavi, and W. Z. Yang Use of Exogenous Fibrolytic Enzymes to Improve Feed Utilization by Ruminants J Anim Sci, February 1, 2003; 81(14_suppl_2): E37 - 47. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. R. Bowman, K. A. Beauchemin, and J. A. Shelford The Proportion of the Diet to which Fibrolytic Enzymes are Added Affects Nutrient Digestion by Lactating Dairy Cows J Dairy Sci, December 1, 2002; 85(12): 3420 - 3429. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Kung Jr., M. A. Cohen, L. M. Rode, and R. J. Treacher The Effect of Fibrolytic Enzymes Sprayed onto Forages and Fed in a Total Mixed Ratio to Lactating Dairy Cows J Dairy Sci, September 1, 2002; 85(9): 2396 - 2402. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
