J. Anim Sci.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Zhang, Y. L.
Right arrow Articles by Davis, D. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Zhang, Y. L.
Right arrow Articles by Davis, D. L.

Journal of Animal Science, Vol 78, Issue 1 131-138, Copyright © 2000 by American Society of Animal Science

JOURNAL ARTICLE

Morphology of luminal and glandular epithelial cells from pig endometrium grown on plastic or extracellular matrices

Y. L. Zhang and D. L. Davis
Department of Animal Sciences and Industry, Kansas State University, Manhattan 66506-0201, USA.

Luminal (LE) and glandular epithelial (GE) cells from d-13 pregnant pigs were cultured on plastic, matrix secreted from endometrial stroma, and EHS matrix (Matrigel) in culture medium (RPMI-1640) supplemented with 20 and 10% fetal bovine serum, respectively. After culture for 7 and 14 d, GE and LE cells were prepared for transmission and GE cells for scanning electron microscopy. The two types of endometrial epithelial cells displayed different morphological characteristics when grown on different culture substrates. On plastic, the GE and LE cells formed flattened monolayers. However, stroma-secreted matrix directed the polarization of endometrial epithelia. The GE and LE cells reacted differently to thick Matrigel coatings; LE cells formed a colony after 7 d of culture and then proliferated further to form a colony with a cavity, but GE cells organized to form a colony with a shallow depression in the center at 7 d and developed duct-like structures after 14 d in vitro. Luminal epithelial cells grown on either diluted or thin-coated Matrigel and grown on stroma-secreted matrix formed a monolayer but no three-dimensional structures.


This article has been cited by other articles:


Home page
 Infect. Immun.Home page
N. V. Guseva, S. T. Knight, J. D. Whittimore, and P. B. Wyrick
Primary Cultures of Female Swine Genital Epithelial Cells In Vitro: a New Approach for the Study of Hormonal Modulation of Chlamydia Infection
Infect. Immun., August 1, 2003; 71(8): 4700 - 4710.
[Abstract] [Full Text] [PDF]

Home page
 J ANIM SCIHome page
P. G. Groothuis, W. J. McGuire, J. L. Vallet, D. M. Grieger, and D. L. Davis
Retinol and estradiol regulation of retinol binding protein and prostaglandin production by porcine uterine epithelial cells in vitro
J Anim Sci, October 1, 2002; 80(10): 2688 - 2694.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2000 by the American Society of Animal Science.