Journal of Animal Science, Vol 77, Issue 5 1277-1283, Copyright © 1999 by American Society of Animal Science

JOURNAL ARTICLE

Tissue distribution of a peptide transporter mRNA in sheep, dairy cows, pigs, and chickens

H. Chen, E. A. Wong and K. E. Webb Jr
Department of Animal and Poultry Sciences, Virginia Polytechnic Institute and State University, Blacksburg 24061-0306, USA.

A 446-bp cDNA fragment encoding a peptide transport protein was cloned from sheep omasum and used as a probe to study the distribution of the peptide transport protein mRNA in various tissues of sheep, dairy cows, pigs, and chickens. Because the predicted amino acid sequence of this fragment was 85.8, 90.5, and 90.5% identical to rabbit, human, and rat intestinal peptide transporter (PepT1), respectively, it is believed that this cloned fragment represents PepT1 from sheep. In sheep (n = 5) and lactating Holstein cows (n = 3), hybridization was observed with mRNA from the omasum, rumen, duodenum, jejunum, and ileum. The estimated size of mRNA was 2.8 kb. No hybridization was observed with mRNA from the abomasum, cecum, colon, liver, kidney, and semitendinosus and longissimus muscles of either species or the mammary gland of the dairy cows. In pigs (n = 6), the probe hybridized with mRNA from the duodenum, jejunum, and ileum. There was no hybridization with mRNA from the stomach, large intestine, liver, kidney, and semitendinosus and longissimus muscles. Two bands, 3.5 and 2.9 kb, were observed with northern blot analysis, indicating two RNA transcripts that may result from alternative mRNA processing. In White Leghorns (n = 15) and broilers (n = 20), the strongest hybridization was found in the duodenum, but the jejunum and ileum showed faint bands. The size of mRNA in chickens was 1.9 kb. Other tissues, including the crop, proventriculus, gizzard, ceca, liver, kidney, and muscles showed no hybridization to the probe. In conclusion, mRNA for PepT1 is present in the small intestine of all animals examined and the omasal and ruminal epithelium of sheep and dairy cows. The size of the mRNA varied among species.

This article has been cited by other articles:

				E. R. Gilbert, E. A. Wong, and K. E. Webb Jr. BOARD-INVITED REVIEW: Peptide absorption and utilization: Implications for animal nutrition and health J Anim Sci, September 1, 2008; 86(9): 2135 - 2155. [Abstract] [Full Text] [PDF]

				H. Tagari, K. Webb Jr, B. Theurer, T. Huber, D. DeYoung, P. Cuneo, J. E. P. Santos, J. Simas, M. Sadik, A. Alio, et al. Mammary Uptake, Portal-Drained Visceral Flux, and Hepatic Metabolism of Free and Peptide-Bound Amino Acids in Cows Fed Steam-Flaked or Dry-Rolled Sorghum Grain Diets J Dairy Sci, February 1, 2008; 91(2): 679 - 697. [Abstract] [Full Text] [PDF]

				E. R. Gilbert, H. Li, D. A. Emmerson, K. E. Webb Jr, and E. A. Wong Dietary Protein Quality and Feed Restriction Influence Abundance of Nutrient Transporter mRNA in the Small Intestine of Broiler Chicks J. Nutr., February 1, 2008; 138(2): 262 - 271. [Abstract] [Full Text] [PDF]

				E. R. Gilbert, H. Li, D. A. Emmerson, K. E. Webb Jr., and E. A. Wong Developmental Regulation of Nutrient Transporter and Enzyme mRNA Abundance in the Small Intestine of Broilers Poult. Sci., August 1, 2007; 86(8): 1739 - 1753. [Abstract] [Full Text] [PDF]

				S. J. Mabjeesh, O. Gal-Garber, J. Milgram, Y. Feuermann, M. Cohen-Zinder, and A. Shamay Aminopeptidase N Gene Expression and Abundance in Caprine Mammary Gland is Influenced by Circulating Plasma Peptide J Dairy Sci, June 1, 2005; 88(6): 2055 - 2064. [Abstract] [Full Text] [PDF]

				J. E. Klang, L. A. Burnworth, Y. X. Pan, K. E. Webb Jr., and E. A. Wong Functional characterization of a cloned pig intestinal peptide transporter (pPepT1) J Anim Sci, January 1, 2005; 83(1): 172 - 181. [Abstract] [Full Text] [PDF]

				H. Tagari, K. Webb Jr., B. Theurer, T. Huber, D. DeYoung, P. Cuneo, J. E. P. Santos, J. Simas, M. Sadik, A. Alio, et al. Portal Drained Visceral Flux, Hepatic Metabolism, and Mammary Uptake of Free and Peptide-Bound Amino Acids and Milk Amino Acid Output in Dairy Cows Fed Diets Containing Corn Grain Steam Flaked at 360 or Steam Rolled at 490 g/L J Dairy Sci, February 1, 2004; 87(2): 413 - 430. [Abstract] [Full Text] [PDF]

				A. J. Sweatt, M. Wood, A. Suryawan, R. Wallin, M. C. Willingham, and S. M. Hutson Branched-chain amino acid catabolism: unique segregation of pathway enzymes in organ systems and peripheral nerves Am J Physiol Endocrinol Metab, January 1, 2004; 286(1): E64 - E76. [Abstract] [Full Text]

				H. Chen, Y. Pan, E. A. Wong, J. R. Bloomquist, and K. E. Webb Jr. Molecular Cloning and Functional Expression of a Chicken Intestinal Peptide Transporter (cPepT1) in Xenopus Oocytes and Chinese Hamster Ovary Cells J. Nutr., March 1, 2002; 132(3): 387 - 393. [Abstract] [Full Text] [PDF]

				Y. Pan, E. A. Wong, J. R. Bloomquist, and K. E. Webb Jr. Expression of a Cloned Ovine Gastrointestinal Peptide Transporter (oPepT1) in Xenopus Oocytes Induces Uptake of Oligopeptides in Vitro J. Nutr., April 1, 2001; 131(4): 1264 - 1270. [Abstract] [Full Text]

				C. C. Metges Contribution of Microbial Amino Acids to Amino Acid Homeostasis of the Host J. Nutr., July 1, 2000; 130(7): 1857S - 1864. [Abstract] [Full Text]