J. Anim Sci.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wampler, J. L.
Right arrow Articles by Hill, G. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wampler, J. L.
Right arrow Articles by Hill, G. M.

Journal of Animal Science, Vol 76, Issue 10 2730-2736, Copyright © 1998 by American Society of Animal Science

JOURNAL ARTICLE

Effects of laidlomycin propionate and monensin on glucose utilization and nutrient transport by Streptococcus bovis and Selenomonas ruminantium

J. L. Wampler, S. A. Martin and G. M. Hill
Department of Animal and Dairy Science, University of Georgia, Athens 30602-2771, USA.

The objective of this study was to compare the effects of laidlomycin propionate and monensin on cell growth, glucose fermentation, and glucose uptake in Streptococcus bovis strain JB1 and Selenomonas ruminantium strain HD4. Experiments were also conducted to compare the effects of both ionophores on sodium-dependent serine transport and cell yield in S. bovis. Batch cultures (500 mL) of each bacterium were grown on 3.6 g/L D-glucose in semidefined medium and treated with either 5 ppm monensin or 2 ppm laidlomycin propionate (n=2). Cell growth was monitored by measuring optical density at 600 nm (OD600). Glucose and L-lactate concentrations were measured using coupled enzyme assays. In S. bovis, both monensin and laidlomycin propionate decreased OD600, glucose utilization, and L-lactate production. Neither ionophore had any effect on glucose utilization by S. ruminantium. [14C]Glucose uptake between 5 and 30 min by both bacteria was not altered by either ionophore. Sodium-dependent [14C]serine uptake by S. bovis was inhibited by monensin but not laidlomycin propionate. When S. bovis was grown in glucose-limited continuous culture (dilution rate=.10 h(-1)) at extracellular pH 6.7, increasing concentrations of both ionophores decreased bacterial yield, and both ionophores were more potent at an extracellular pH of 5.7. However, monensin was a more potent inhibitor than laidlomycin propionate at pH 6.7 and 5.7. Collectively, these results suggest that the ionophore laidlomycin propionate inhibits the Gram-positive bacterium S. bovis in a manner similar to that of monensin, but, at the concentrations used in this study, laidlomycin propionate seems to be less potent than monensin in inhibiting serine uptake and cell yield.


This article has been cited by other articles:


Home page
 J ANIM SCIHome page
Y. K. Fan, J. Croom, E. J. Eisen, H. R. Spires, and L. R. Daniel
Ionophores have limited effects on jejunal glucose absorption and energy metabolism in mice
J Anim Sci, August 1, 2003; 81(8): 2072 - 2079.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1998 by the American Society of Animal Science.