J. Anim Sci.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Abdelgadir, I. E.
Right arrow Articles by Vanzant, E. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Abdelgadir, I. E.
Right arrow Articles by Vanzant, E. S.

Journal of Animal Science, Vol 75, Issue 8 2215-2222, Copyright © 1997 by American Society of Animal Science

JOURNAL ARTICLE

In vitro determination of ruminal protein degradability of alfalfa and prairie hay via a commercial protease in the presence or absence of cellulase or driselase

I. E. Abdelgadir, R. C. Cochran, E. C. Titgemeyer and E. S. Vanzant
Department of Animal Sciences and Industry, Kansas State University, Manhattan 66506, USA.

Ruminal protein degradation of alfalfa (2.62% N, 49.6% NDF, and in vivo undegradable intake protein [UIP] = 16.4% of CP) and prairie hay (.88% N, 69.4% NDF, and in vivo UIP = 44.5% of CP) was estimated using the Streptomyces griseus protease (SGP) in vitro method with or without pretreatment with two carbohydrases: cellulase from Penicillium funiculosum or driselase from Basidiomycetes. Driselase is a broad-spectrum carbohydrase. Incubating forage samples for 48 h with cellulase or driselase at a concentration of 800 mg/g per g of hay nearly maximized ADF and NDF disappearances. This concentration and incubation time then were used to pretreat hay samples. A 2-h pretreatment was included to evaluate the potential for reducing the analysis time. Other sets of samples were or were not pretreated with acetate buffer alone. Following pretreatment, samples were subjected to SGP for .25, .5, 1, 2, 4, 8, 12, 24, and 48 h. Pretreatment altered the sizes of protein pools and their degradation rates. When the UIP contents of the forages were estimated using SGP and a single-pool, first-order, kinetic model, cellulase (48 h) or driselase pretreatments yielded UIP predictions that were more similar to in vivo values. Some carbohydrase and protease combinations also yielded single time-point estimates of UIP that were similar to in vivo values. Similarly, when sufficient time was permitted for protease incubation, single time-point estimates derived from protease alone were similar to in vivo values.


This article has been cited by other articles:


Home page
 Crop Sci.Home page
W. K. Coblentz, J. E. Turner, D. A. Scarbrough, K. P. Coffey, D. W. Kellogg, and L. J. McBeth
Effects of Spontaneous Heating on Estimates of Ruminal Nitrogen Degradation in Bermudagrass Hays from Two Harvests
Crop Sci., September 1, 2001; 41(5): 1572 - 1578.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1997 by the American Society of Animal Science.