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Journal of Animal Science, Vol 75, Issue 8 2161-2164, Copyright © 1997 by American Society of Animal Science

JOURNAL ARTICLE

Technical note: pig model for studying nutrient assimilation by the intestine and colon

C. L. Kien, A. H. Ailabouni, R. D. Murray, P. A. Powers, R. E. McClead and J. Kepner
Department of Pediatrics, Ohio State University College of Medicine and Children's Hospital, Columbus 43205, USA.

We have developed a system for chronically catheterizing 10- to 25-d-old pigs that permits stable isotope tracer studies of intestinal or colonic assimilation of nutrients. This model also can be used to ensure constant enteral feeding or to assess the rate of entry into the terminal ileum of carbohydrates, fats, and amino acids. A plastic cannula with a luminal flange can be surgically placed in the stomach for tracer studies of sugar digestion or for controlled infusion of any formula diet. A similar cannula can be placed in the cecum for infusion of tracer and(or) substrates for studies of fermentation. The cannula has been machined so that a washer and nut can be threaded onto it, allowing the entire apparatus to be fixed to the abdominal wall. The distal end protruding above the skin was tapered to fit standard i.v. extension tubing. A carotid arterial catheter was used to sample substrates for isotopic enrichment measurements.


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Copyright © 1997 by the American Society of Animal Science.