J. Anim Sci.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Reecy, J. M.
Right arrow Articles by Davis, J. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Reecy, J. M.
Right arrow Articles by Davis, J. L.

Journal of Animal Science, Vol 74, Issue 9 2158-2169, Copyright © 1996 by American Society of Animal Science

JOURNAL ARTICLE

The effect of postruminal amino acid flow on muscle cell proliferation and protein turnover

J. M. Reecy, J. E. Williams, M. S. Kerley, R. S. MacDonald, W. H. Thornton Jr and J. L. Davis
Department of Animal Science, University of Missouri, Columbia 65211, USA.

An experiment was conducted to characterize the effects of postruminal administration of casein, glutamine, cornstarch, and water on protein turnover and in vitro muscle cell proliferation. Four MARC III steers (205 kg) were fed a protein-restricted bromegrass hay-based diet (2.86 Mcal of DE/kg and 13.6 g of N/kg). Using a 4 x 5 Latin square arrangement balanced for residual effects, casein and glutamine, equal to 50% of basal dietary nitrogen intake, cornstarch, isocaloric with casein infusion, or an equal volume of water was continuously infused into the abomasum of steers. Blood samples, collected every 2 h for 24 h after 7 d of infusion, were tested for the effect on cell cycle kinetics and myotube protein turnover. Urine and feces were also collected for 4 d after blood sampling for nitrogen balance and fractional skeletal muscle degradation. The mitogenic activity and ability of serum to influence rate of myoblast proliferation in a dose-dependent manner was influenced (P < .05) by infusate: casein > cornstarch > glutamine = water. Abomasal infusion of casein and cornstarch increased (P < .05) in vitro muscle protein synthesis and decreased (P < .05) in vitro muscle protein degradation, whereas abomasal glutamine infusion only increased (P < .05) in vitro muscle protein synthesis. Abomasal glutamine infusion decreased (P < .05) fractional skeletal muscle protein degradation and synthesis; however, fractional muscle protein accretion tended to increase due to a greater decline in fractional muscle protein degradation. In contrast, abomasal casein infusion increased (P < .05) fractional skeletal muscle protein synthesis, breakdown, and accretion. These results suggest that muscle hypertrophy may be regulated by serum constituents whose activity is affected by postruminal amino acid flow.


This article has been cited by other articles:


Home page
 J DAIRY SCIHome page
L. Doepel, M. Lessard, N. Gagnon, G. E. Lobley, J. F. Bernier, P. Dubreuil, and H. Lapierre
Effect of postruminal glutamine supplementation on immune response and milk production in dairy cows.
J Dairy Sci, August 1, 2006; 89(8): 3107 - 3121.
[Abstract] [Full Text] [PDF]

Home page
 J. Nutr.Home page
G. E. Lobley, S. O. Hoskin, and C. J. McNeil
Glutamine in Animal Science and Production
J. Nutr., September 1, 2001; 131(9): 2525S - 2531.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1996 by the American Society of Animal Science.