| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Journal of Animal Science, Vol 74, Issue 5 975-983, Copyright © 1996 by American Society of Animal Science
JOURNAL ARTICLE |
D. R. Smith, D. A. Knabe and S. B. Smith
Department of Animal Science, Texas Agricultural Experiment Station, Texas A&M University, College Station 77843-2471, USA.
The objective of this study was to document the influence of specific dietary fatty acids on rates of lipid synthesis and sensitivity to insulin in porcine adipose tissue. Weanling pigs were assigned to one of six groups, and each group was fed diets containing 10 g/100 g of added cornstarch or 10 g/100 g of added fatty acid. The fatty acid-enriched diets contained either a combination of 14:1 plus 16:1 (14:1/16:1 diet), 16:0, 18:0, 18:1, or 18:2 (n-6). With the exception of the cornstarch diet, all diets contained approximately 35% 14:0. Subcutaneous adipose tissue samples were collected at slaughter from the area overlying the first cranial vertebra. Fresh samples were incubated for 2 h in 20 mM glucose and 0, 10, 100 or 1,000 microU/mL of porcine insulin. The smallest adipocytes were observed in adipose tissue from pigs fed the 16:0 or 18:2 diets. Glucose incorporation into lipids was greater (P < .05) in adipose tissue from cornstarch-fed pigs than in adipose tissue from the other treatment groups. Lipogenesis was 67, 53, 35, 32, and 20% lower (P < .05) in adipose tissue from 16:0-, 14:1/16:1-, 18:0-, 18:2-, and 18:1-fed pigs, respectively, than in adipose tissue from the cornstarch-fed pigs. Insulin increased lipogenesis by 19% (P < .05) in adipose tissue from the cornstarch-fed pigs and by 15 to 40% (P < .05) in adipose tissue from the 14:1/16:1-fed pigs. Insulin did not stimulate lipogenesis (P > .4) in adipose tissue from pigs fed the 16:0, 18:0, or 18:1 diets. The data suggest that fatty acid chain length and unsaturation are determinants in the effects of dietary fat and insulin on de novo lipogenesis.
This article has been cited by other articles:
|
|
 |
|
  A. R. Jackson, S. Powell, S. L. Johnston, J. O. Matthews, T. D. Bidner, F. R. Valdez, and L. L. Southern The effect of chromium as chromium propionate on growth performance, carcass traits, meat quality, and the fatty acid profile of fat from pigs fed no supplemented dietary fat, choice white grease, or tallow J Anim Sci, December 1, 2009; 87(12): 4032 - 4041. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. D. Rhoades, J. E. Sawyer, C. H. Ponce, D. K. Lunt, and S. B. Smith Substrate utilization and dose response to insulin by subcutaneous adipose tissue of Angus steers fed corn- or hay-based diets J Anim Sci, July 1, 2009; 87(7): 2338 - 2345. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. L. Nall, G. Wu, K. H. Kim, C. W. Choi, and S. B. Smith Dietary Supplementation of L-Arginine and Conjugated Linoleic Acid Reduces Retroperitoneal Fat Mass and Increases Lean Body Mass in Rats J. Nutr., July 1, 2009; 139(7): 1279 - 1285. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. J. Hausman, M. V. Dodson, K. Ajuwon, M. Azain, K. M. Barnes, L. L. Guan, Z. Jiang, S. P. Poulos, R. D. Sainz, S. Smith, et al. BOARD-INVITED REVIEW: The biology and regulation of preadipocytes and adipocytes in meat animals J Anim Sci, April 1, 2009; 87(4): 1218 - 1246. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. D. Rhoades, J. E. Sawyer, K. Y. Chung, M. L. Schell, D. K. Lunt, and S. B. Smith Effect of dietary energy source on in vitro substrate utilization and insulin sensitivity of muscle and adipose tissues of Angus and Wagyu steers J Anim Sci, July 1, 2007; 85(7): 1719 - 1726. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. B. Smith, A. A. Chapman, D. K. Lunt, J. J. Harris, and J. W. Savell Adiposity of calf- and yearling-fed Brangus steers raised to constant-age and constant-body weight endpoints J Anim Sci, May 1, 2007; 85(5): 1136 - 1143. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. G. Bergen and H. J. Mersmann Comparative Aspects of Lipid Metabolism: Impact on Contemporary Research and Use of Animal Models J. Nutr., November 1, 2005; 135(11): 2499 - 2502. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. L. Archibeque, D. K. Lunt, C. D. Gilbert, R. K. Tume, and S. B. Smith Fatty acid indices of stearoyl-CoA desaturase do not reflect actual stearoyl-CoA desaturase enzyme activities in adipose tissues of beef steers finished with corn-, flaxseed-, or sorghum-based diets J Anim Sci, May 1, 2005; 83(5): 1153 - 1166. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. J. Azain Role of fatty acids in adipocyte growth and development J Anim Sci, March 1, 2004; 82(3): 916 - 924. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S.-T. Ding, A. Lapillonne, W. C. Heird, and H. J. Mersmann Dietary fat has minimal effects on fatty acid metabolism transcript concentrations in pigs J Anim Sci, February 1, 2003; 81(2): 423 - 431. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. R. Demaree, C. D. Gilbert, H. J. Mersmann, and S. B. Smith Conjugated Linoleic Acid Differentially Modifies Fatty Acid Composition in Subcellular Fractions of Muscle and Adipose Tissue but Not Adiposity of Postweanling Pigs J. Nutr., November 1, 2002; 132(11): 3272 - 3279. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
