Journal of Animal Science, Vol 74, Issue 3 693-700, Copyright © 1996 by American Society of Animal Science

JOURNAL ARTICLE

Effects of varying levels of duodenal or midjejunal glucose and 2-deoxyglucose infusion on small intestinal disappearance and net portal glucose flux in steers

C. R. Krehbiel, R. A. Britton, D. L. Harmon, J. P. Peters, R. A. Stock and H. E. Grotjan
University of Nebraska, Lincoln 68583, USA.

Six crossbred steers (261 +/- 18 kg BW) fitted with hepatic portal, mesenteric venous and arterial catheters, and duodenal, midjejunal, and ileal cannulas were used in a replicated 3 x 3 Latin square design to determine the effect of varying levels and site of glucose plus 2-deoxyglucose (2DG) infusion on net portal-drained visceral flux. Steers were fed chopped alfalfa in six equal portions daily at 1.5% of BW. Glucose (0, 9, or 18 g/h) and 2DG (0, 1, or 2 g/h) were infused continuously through the duodenal or midjejunal cannula (two infusion sites) at total glucose plus 2DG infusion rates of 0, 10, or 20 g/h. Arterial and portal blood samples were taken simultaneously at 20-min intervals from 5 to 9 h of infusion. Portal blood flow was determined by continuous infusion of p-aminohippurate and net flux was calculated as venous-arterial concentration (PA) difference times blood flow. Arterial concentration of glucose was not affected (P > .10) by glucose plus 2DG infusion, whereas arterial concentration of 2DG was greater (P < .05) when glucose plus 2DG was infused into the duodenum and increased (linear, P < .10) as amount of glucose plus 2DG infused into both the duodenum and midjejunum increased. Net portal flux and PA difference of glucose and 2DG were greater (P < .05) when glucose plus 2DG was infused into the duodenum. Although 2DG was infused at 10% of the total glucose plus 2DG infusion, it accounted for only 1.7 and .7% of the glucose plus 2DG appearing in portal blood when glucose plus 2DG was infused at 10 and 20 g/h, respectively. We conclude that glucose is more readily absorbed across the proximal-half than the distal-half of the small intestine, and that passive diffusion is a minor route of glucose absorption.

This article has been cited by other articles:

				M. Larsen and N. B. Kristensen Effect of abomasal glucose infusion on splanchnic and whole-body glucose metabolism in periparturient dairy cows J Dairy Sci, March 1, 2009; 92(3): 1071 - 1083. [Abstract] [Full Text] [PDF]

				C. Loncke, I. Ortigues-Marty, J. Vernet, H. Lapierre, D. Sauvant, and P. Noziere Empirical prediction of net portal appearance of volatile fatty acids, glucose, and their secondary metabolites ({beta}-hydroxybutyrate, lactate) from dietary characteristics in ruminants: A meta-analysis approach J Anim Sci, January 1, 2009; 87(1): 253 - 268. [Abstract] [Full Text] [PDF]

				S. M. Rodriguez, K. C. Guimaraes, J. C. Matthews, K. R. McLeod, R. L. Baldwin VI, and D. L. Harmon Influence of abomasal carbohydrates on small intestinal sodium-dependent glucose cotransporter activity and abundance in steers J Anim Sci, October 1, 2004; 82(10): 3015 - 3023. [Abstract] [Full Text] [PDF]

				J. R. Aschenbach, S. K. Bhatia, H. Pfannkuche, and G. Gabel Glucose Is Absorbed in a Sodium-Dependent Manner from Forestomach Contents of Sheep J. Nutr., November 1, 2000; 130(11): 2797 - 2801. [Abstract] [Full Text] [PDF]

				J. P. Cant, P. H. Luimes, T. C. Wright, and B. W. McBride Modeling intermittent digesta flow to calculate glucose uptake capacity of the bovine small intestine Am J Physiol Gastrointest Liver Physiol, June 1, 1999; 276(6): G1442 - G1451. [Abstract] [Full Text] [PDF]