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Journal of Animal Science, Vol 74, Issue 10 2406-2410, Copyright © 1996 by American Society of Animal Science

JOURNAL ARTICLE

Technical note: a simplified procedure for vitamin E determination in beef muscle

Q. Liu, K. K. Scheller and D. M. Schaefer
Department of Meat and Animal Science, University of Wisconsin-Madison 53706-1284, USA.

A simplified method for the determination of alpha-tocopherol concentration in beef muscle was developed and evaluated. The method consists of a saponification step applied to 1-g samples of intact, fresh muscle, followed by a single isooctane extraction of the saponified samples. alpha-Tocopherol in the extract was separated by normal phase chromatography and quantified by fluorescence detection. A single extraction with the simplified method accounted for 95% of the total muscle alpha-tocopherol concentration obtained by two extractions with the Arnold et al. (J. Food Sci. 58:28, 1993) method. Recovery of added alpha-tocopherol standard after two extractions of a saponified muscle sample was 91% for the simplified method, which was not different (alpha = .78) from recovery using the Arnold method, and the efficiency of the single extraction in the simplified method was 89%. The coefficients of variation for the simplified and Arnold methods were both 3.1%. This method should permit the duplicate analysis of 100 fresh muscle samples within 24 h.


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Copyright © 1996 by the American Society of Animal Science.