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Journal of Animal Science, Vol 73, Issue 3 785-792, Copyright © 1995 by American Society of Animal Science
JOURNAL ARTICLE |
R. O. Ball, E. S. Batterham and R. J. van Barneveld
Department of Animal and Poultry Science, University of Guelph, Ontario, Canada.
Knowledge of amino acid availability in feedstuffs is central to accurate diet formulation. Dietary lysine oxidation was evaluated as a means of predicting dietary lysine availability. Growing pigs (30 kg) were offered control (1.43% lysine), free lysine-supplemented soybean meal-, or cottonseed meal-supplemented diets. Supplemented diets provided equivalent total lysine (approximately 24.2 g/d at 30 kg BW) but availabilities of lysine, determined by slope-ratio assay, in the free lysine, soybean meal, and cottonseed meal were 100, 90, and 30%, respectively. Feed was offered in eight equally spaced meals per day to achieve three times maintenance energy intake. Following a meal containing L-[U-14C]lysine (1 mu Ci/kg BW), lysine oxidation, as 14CO2 expired, was lower (P < .05) for the control diet but not different between the other three diets, contrary to the hypothesis. Lysine oxidation following an intravenous bolus dose was lowest (P < .05) for pigs fed the control, highest (P < .05) for pigs fed the cottonseed diet, and intermediate (P < .05) for pigs fed the free lysine-supplemented diet (27.1, 80.2, and 47.5, dpm/kg x 10(-2), respectively). Plasma lysine concentration was lower (P < .05) and lysine specific radioactivity tended to be higher (P < .10) following a meal containing cottonseed than following a meal containing free lysine, indicating that the lysine pool was smaller in pigs receiving the cottonseed-meal diet.(ABSTRACT TRUNCATED AT 250 WORDS)
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