Journal of Animal Science, Vol 73, Issue 2 541-545, Copyright © 1995 by American Society of Animal Science

JOURNAL ARTICLE

An increase in serum lipids increases luteal lipid content and alters the disappearance rate of progesterone in cows

D. E. Hawkins, K. D. Niswender, G. M. Oss, C. L. Moeller, K. G. Odde, H. R. Sawyer and G. D. Niswender
Department of Physiology, Colorado State University, Fort Collins 80523, USA.

To determine whether an increase in serum lipids alters the area occupied by lipid droplets in steroidogenic luteal cells and(or) clearance rates of progesterone from serum, pregnant beef heifers received control (n = 6) or treatment (n = 5) diets. To increase serum lipids, the treatment diet contained calcium soaps of fatty acids. Control and treatment diets were formulated to be isocaloric and isonitrogenous. Feeding of diets was initiated approximately 100 d before parturition and continued through the third postpartum estrous cycle. On d 12 or 13 of the third postpartum cycle, corpora lutea were collected by ovariectomy and a center slice was processed for electron microscopy. Eight samples from each slice were sectioned, stained, and examined at a magnification of 2,500x. Five micrographs per sample were analyzed for area occupied by small (SLC) and large (LLC) luteal cells, percentage of the area of each steroidogenic cell type occupied by lipid, and total steroidogenic area (SLC + LLC) occupied by lipid. Jugular blood was collected before and after ovariectomy, and progesterone, cholesterol, high-density lipoprotein (HDL), and low-density lipoprotein (LDL) were quantified. Cows consuming treatment diets had approximately twice (P < .05) the concentration of cholesterol, HDL, and progesterone in serum that controls had. The percentage of the area of SLC, LLC, and total area occupied by lipid was greater (P < .05) in treated than in control cows. The average time required for serum concentrations of progesterone to decrease by 50% after ovariectomy was greater (P < .05) in treated than in control cows (170 +/- 16 vs 113 +/- 15 min).(ABSTRACT TRUNCATED AT 250 WORDS)

This article has been cited by other articles:

				C. N. Lopes, A. B. Scarpa, B. I. Cappellozza, R. F. Cooke, and J. L. M. Vasconcelos Effects of rumen-protected polyunsaturated fatty acid supplementation on reproductive performance of Bos indicus beef cows J Anim Sci, December 1, 2009; 87(12): 3935 - 3943. [Abstract] [Full Text] [PDF]

				E. J. Scholljegerdes, B. W. Hess, M. H. J. Grant, S. L. Lake, B. M. Alexander, T. R. Weston, D. L. Hixon, E. A. Van Kirk, and G. E. Moss Effects of feeding high-linoleate safflower seeds on postpartum reproduction in beef cows J Anim Sci, September 1, 2009; 87(9): 2985 - 2995. [Abstract] [Full Text] [PDF]

				C. O. Lemley, S. T. Butler, W. R. Butler, and M. E. Wilson Short Communication: Insulin Alters Hepatic Progesterone Catabolic Enzymes Cytochrome P450 2C and 3A in Dairy Cows J Dairy Sci, February 1, 2008; 91(2): 641 - 645. [Abstract] [Full Text] [PDF]

				M.-H. Wu, K.-F. Chen, S.-C. Lin, C.-W. Lgu, and S.-J. Tsai Aberrant Expression of Leptin in Human Endometriotic Stromal Cells Is Induced by Elevated Levels of Hypoxia Inducible Factor-1{alpha} Am. J. Pathol., February 1, 2007; 170(2): 590 - 598. [Abstract] [Full Text] [PDF]

				T. R. Bilby, J. Block, B. C. do Amaral, O. Sa Filho, F. T. Silvestre, P. J. Hansen, C. R. Staples, and W. W. Thatcher Effects of dietary unsaturated Fatty acids on oocyte quality and follicular development in lactating dairy cows in summer. J Dairy Sci, October 1, 2006; 89(10): 3891 - 3903. [Abstract] [Full Text] [PDF]

				S J Adamiak, K Powell, J A Rooke, R Webb, and K D Sinclair Body composition, dietary carbohydrates and fatty acids determine post-fertilisation development of bovine oocytes in vitro Reproduction, February 1, 2006; 131(2): 247 - 258. [Abstract] [Full Text] [PDF]

				R. N. Funston Fat supplementation and reproduction in beef females J Anim Sci, January 1, 2004; 82(13_suppl): E154 - 161. [Abstract] [Full Text] [PDF]

				T. Kassa, J. D. Ambrose, A. L. Adams, C. Risco, C. R. Staples, M.-J. Thatcher, H. H. Van Horn, A. Garcia, H. H. Head, and W. W. Thatcher Effects of Whole Cottonseed Diet and Recombinant Bovine Somatotropin on Ovarian Follicles in Lactating Dairy Cows J Dairy Sci, November 1, 2002; 85(11): 2823 - 2830. [Abstract] [Full Text] [PDF]