J. Anim Sci.
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Journal of Animal Science, Vol 73, Issue 10 3009-3021, Copyright © 1995 by American Society of Animal Science

JOURNAL ARTICLE

Effects of glycine and bovine serum albumin on inhibition of propionate metabolism in ovine hepatocytes caused by reduced phenolic monomers

J. D. Cremin Jr, J. K. Drackley, L. R. Hansen, D. E. Grum, J. Odle and G. C. Fahey Jr
Department of Animal Sciences, University of Illinois, Urbana 61801, USA.

Hepatocytes isolated from sheep were incubated in the presence of reduced phenolics and glycine to determine the effects of these compounds on hepatic propionate metabolism in vitro. 3-Phenyl-propionic (PPA) or t-cinnamic (CA) acids, but not benzoic (BA) or 3-(4-hydroxyphenyl)propionic (4OHPPA) acids, decreased conversion of propionate to glucose at .05 mM in the absence of supplemental glycine. At 1.2 mM, all reduced phenolics decreased conversion of propionate to glucose in the absence of supplemental glycine. Addition of glycine to the incubation medium linearly alleviated the inhibition by BA, PPA, or CA, suggesting that physiological glycine concentrations limited alleviation of inhibition of propionate metabolism. Hippuric acid production increased as glycine concentration increased in the presence of PPA, CA, or 4OHPPA. Bovine serum albumin did not alleviate inhibition of conversion of propionate to glucose caused by BA, PPA, or CA and slightly alleviated inhibition caused by 4OHPPA (.4 mM). Of the reduced phenolics tested, PPA is the most likely to inhibit gluconeogenesis from propionate in ovine liver in vivo.




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Copyright © 1995 by the American Society of Animal Science.