J. Anim Sci.
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Journal of Animal Science, Vol 72, Issue 9 2378-2384, Copyright © 1994 by American Society of Animal Science

JOURNAL ARTICLE

A microplate-based bioassay system for measuring porcine serum mitogenic activity

W. Zhou, E. T. Kornegay and B. Storrie
Department of Animal Science, Virginia Polytechnic Institute and State University, Blacksburg 24061-0306.

A bioassay system was developed to measure porcine serum mitogenic activity, which is an indicator of growth factor activity. A MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazoliumbromide++ +] cell proliferation assay was used to monitor cell growth. In comparison with electronic cell counting, this bioassay system is sensitive, economical, and semi-automatic. A non-fusing myoblast cell line, BC3H1, was used, making it possible to increase seeding density and thus improve precision. The assay was carried out in 96-well microplates with automatic reading and data processing by a computer-controlled ELISA reader. Results were highly correlated with Kotts' method. This assay system is of potential value in studying growth regulation in pigs.




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Copyright © 1994 by the American Society of Animal Science.