J. Anim Sci.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hathaway, M. R.
Right arrow Articles by Dayton, W. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hathaway, M. R.
Right arrow Articles by Dayton, W. R.

Journal of Animal Science, Vol 72, Issue 8 2001-2007, Copyright © 1994 by American Society of Animal Science

JOURNAL ARTICLE

Transforming growth factor beta-1 facilitates establishing clonal populations of ovine muscle satellite cells

M. R. Hathaway, M. S. Pampusch, J. R. Hembree and W. R. Dayton
Department of Animal Science, University of Minnesota, St. Paul 55108.

Myogenic cells isolated from lamb fetuses (approximately mid-gestation) exhibited a concentration-dependent decrease in myogenic cell proliferation in response to transforming growth factor (TGF) beta-1 (P < .001). Half-maximal inhibition of proliferation occurred at approximately .05 ng of TGF beta-1/mL and maximal inhibition of proliferation occurred at approximately .1 ng of TGF beta-1/mL. The specificity of this inhibition was confirmed by neutralization of the activity following exposure to a TGF beta antibody. The TGF beta-1 also suppressed proliferation of ovine satellite cells isolated from 5-d-old lambs (P < .0035), but to a lesser extent than observed for embryonic cells. In contrast, TGF beta-1 did not significantly suppress serum-stimulated proliferation of ovine satellite cells isolated from 30- or 150-d-old lambs. Similarly, TGF beta-1 did not suppress proliferation of skeletal muscle fibroblast-like cells isolated from either fetal lambs or 150-d-old lambs. In fact, proliferation of fibroblast-like cells derived from embryonic ovine muscle was enhanced by exposure to TGF beta-1 at all levels tested; however, a concentration-dependent response was not observed. Media transfer experiments showed that conditioning of culture media by postnatally derived cells did not render TGF beta-1 inactive. The studies described in this manuscript suggest that sensitivity of ovine myogenic cells to the antiproliferative effect of TGF-beta may vary with the stage of development.(ABSTRACT TRUNCATED AT 250 WORDS)


This article has been cited by other articles:


Home page
 NeurologyHome page
Y. -W. Chen, K. Nagaraju, M. Bakay, O. McIntyre, R. Rawat, R. Shi, and E. P. Hoffman
Early onset of inflammation and later involvement of TGF{beta} in Duchenne muscular dystrophy
Neurology, September 27, 2005; 65(6): 826 - 834.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
J. M. Oldham, J. A. K. Martyn, M. Sharma, F. Jeanplong, R. Kambadur, and J. J. Bass
Molecular expression of myostatin and MyoD is greater in double-muscled than normal-muscled cattle fetuses
Am J Physiol Regulatory Integrative Comp Physiol, May 1, 2001; 280(5): R1488 - R1493.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1994 by the American Society of Animal Science.