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Journal of Animal Science, Vol 72, Issue 7 1778-1785, Copyright © 1994 by American Society of Animal Science

JOURNAL ARTICLE

Active immunization against prostaglandin F2 alpha: effect of conjugate dose and booster interval on antibody titers and estrous behavior in postpubertal beef heifers

M. A. Crowe, W. J. Enright, D. J. Prendiville, C. A. Morrison and J. F. Roche
Teagasc, Grange Research Centre, Dunsany, Co. Meath, Ireland.

To optimize the prostaglandin F2 alpha (PGF) immunization protocol (conjugate [PGF-human serum albumin; PGF-HSA] dose and immunization regimen) to achieve prolonged suppression of estrous behavior (EB) in beef heifers, 56, 14-mo-old cyclic heifers were assigned (n = 7 per treatment) to eight treatments: 1) 3.3 mg of PGF-HSA on d 0 (single); 2) 3.3 mg of PGF-HSA on d 0 and 28 (booster; B); 3) as (2) except on d 0 and 55; 4) as (2) except on d 0 and 83; and 5 to 8) as in Treatments 1 to 4 except using 10 mg of PGF-HSA. The adjuvant was diethylaminoethyl-dextran, and duration of the experiment was 170 d. Heifers were checked twice daily for EB. A persistent corpus luteum (CL) was considered present when progesterone (P4) was > or = .5 ng/mL for > or = six consecutive samples (every 3 to 4 d). Data were analyzed using ANOVA for a factorial plan. All heifers produced plasma antibody titers (samples every 2 wk) against PGF (peak range: 7 to 84% binding at 1: 1,250). There were no effects (P > .10) of conjugate dose and no interactions between dose and immunization regimen for any variable; therefore, data were combined across dose. Mean and peak titers were greater (P < .05) in heifers in 55- and 83-d B treatments than those in single immunization and 28-d B treatments. Overall, 48/55 heifers formed a persistent CL (41/41 for B heifers). In the single, and 55- and 83-d B treatments, 23/42 heifers formed persistent CL in response to single/primary immunization. There was no difference between immunization regimens in duration (133 +/- 4.4 d) of persistent CL.(ABSTRACT TRUNCATED AT 250 WORDS)


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M.A. Crowe, P. Kelly, M.A. Driancourt, M.P. Boland, and J.F. Roche
Effects of Follicle-Stimulating Hormone With and Without Luteinizing Hormone on Serum Hormone Concentrations, Follicle Growth, and Intrafollicular Estradiol and Aromatase Activity in Gonadotropin-Releasing Hormone-Immunized Heifers
Biol Reprod, January 1, 2001; 64(1): 368 - 374.
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Copyright © 1994 by the American Society of Animal Science.