Journal of Animal Science, Vol 72, Issue 11 2969-2979, Copyright © 1994 by American Society of Animal Science

JOURNAL ARTICLE

Ruminal characteristics, microbial populations, and digestive capabilities of newly weaned, stressed calves

F. L. Fluharty, S. C. Loerch and B. A. Dehority
Department of Animal Science, Ohio Agricultural Research and Development Center.

Eight ruminally fistulated steers, 7 to 8 mo old, were used in a completely randomized, 2 x 2 factorial experiment to determine the effects of energy density and protein source in receiving diets on in situ DM, NDF, and N disappearance, concentrations of ruminal bacteria, protozoa, ammonia, and pH. Two energy densities (1.80 and 1.48 Mcal/kg of NEm) and two protein sources (spray-dried blood meal [SDBM] and soybean meal [SBM]) were compared. Fistulated steers were weaned, transported by truck, and held in a sale barn before their arrival at the feedlot. On d 0 (day of arrival at the feedlot), DMI was 62% of DMI on d 7 after arrival. Overall, feeding a high-energy diet resulted in lower (P < .01) in situ DM disappearance (DMD) of orchardgrass than feeding a low-energy diet at both 24 and 48 h. In situ 24-h DMD averaged 46.6% on d-3 and 41.6% on d 0, whereas 48-h in situ DMD on d -3 and 0 averaged 58.2 and 58.6%, respectively, indicating the ruminal microbial population was not inhibited in its ability to digest available substrate. Additionally, there were no differences (P > .10) in 48-h in situ NDF disappearance between d -3 and 0 (58.8 vs 57.8%), respectively. No differences (P > .10) occurred in the concentration of total bacteria, or cellulolytic bacteria, due to feed and water deprivation. Concentration of total protozoa was lower (P < .05) on d 0 than at any other time. Entodinium averaged 72.5% of genera before weaning, and more than 90% of genera found on all treatments by d 21. Diplodinium and Epidinium percentages tended to decline after weaning. Isotricha concentrations were low and Dasytricha were eliminated after d 7. In conclusion, the concentration of ruminal bacteria and the ability to digest available substrate were not decreased immediately after weaning, trucking, and 24 h of feed and water deprivation.