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Journal of Animal Science, Vol 72, Issue 10 2696-2702, Copyright © 1994 by American Society of Animal Science

JOURNAL ARTICLE

Effects of basic fibroblast growth factor and heparin on follicle-stimulating hormone-induced steroidogenesis by bovine granulosa cells

R. K. Vernon and L. J. Spicer
Department of Animal Science, Oklahoma State University, Stillwater 74078.

The objectives of the present study were to determine the effect of and interaction between basic fibroblast growth factor (bFGF) and heparin, a major bFGF-binding protein, on bovine granulosa cell estradiol and progesterone production. Cells from small (1 to 5 mm) follicles were collected from cattle, cultured for 2 d in medium containing 10% fetal calf serum, and then treated for 1 d with bFGF and(or) heparin in serum-free medium. Treatment with .1 to 10 micrograms/mL of heparin for 1 d had no effect (P > .05), whereas 100 micrograms/mL of heparin inhibited (P < .05) FSH-induced estradiol production by bovine granulosa cells. Treatment with .1 to 1.0 ng/mL of bFGF for 1 d, supplied with human serum albumin as a carrier protein, inhibited (P < .05) FSH-induced estradiol production by bovine granulosa cells, with the greatest inhibition detected at 1.0 ng/mL; coculture with 10 micrograms/mL of heparin did not influence these effects. In comparison, .1 ng/mL of bFGF supplied carrier-free had no effect (P > .05) on FSH-induced estradiol production, whereas 1.0 to 100 ng/mL of carrier-free bFGF inhibited (P < .05) FSH-induced estradiol production, with the greatest inhibition detected at 100 ng/mL. The presence of heparin (10 micrograms/mL) had variable effects on granulosa cell estradiol and progesterone production inhibited by carrier-free bFGF. These results indicate that bFGF may play a significant role in FSH-modulated granulosa cell steroidogenesis during follicular development in cattle.


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Copyright © 1994 by the American Society of Animal Science.