Journal of Animal Science, Vol 71, Issue 5 1247-1255, Copyright © 1993 by American Society of Animal Science

JOURNAL ARTICLE

Effect of copper deficiency on tissue, blood characteristics, and immune function of calves challenged with infectious bovine rhinotracheitis virus and Pasteurella hemolytica

J. R. Stabel, J. W. Spears and T. T. Brown Jr
National Animal Disease Center, ARS, USDA, Ames, IA 50010.

Fourteen Holstein steers, averaging 30 d of age, were fed a semipurified diet (1.5 mg of Cu/kg) supplemented with 0 (-Cu) or 10 mg of Cu/kg of diet (+Cu) for 5 mo. Calves were then challenged by consecutive exposure to aerosol preparations of infectious bovine rhinotracheitis virus (IBRV) and Pasteurella hemolytica on d 0 and 7, respectively, of the 30-d study. Serum ceruloplasmin and plasma copper were higher in +Cu calves throughout the challenge period and increased in +Cu calves after microbial challenge. Heart weights were higher in -Cu calves, although weights of liver, spleen, and thymus were not different between treatments. Copper concentrations in all tissues as well as thymus zinc were higher in +Cu calves. Serum immunoglobulin M tended to be higher in +Cu calves and increased in both treatments after IBRV challenge. Serum IBRV antibody titers were higher in -Cu calves with detectable seroconversion by d 10 postinfection. In contrast, antigen-specific antibodies to P. hemolytica tended to be higher in +Cu calves on d 21. Copper status did not affect blastogenic response, but phytohemagglutinin (PHA)-stimulated blastogenesis was higher in both treatments after IBRV challenge. Repletion of lymphocyte cultures with copper chloride increased proliferative responses to PHA in both +Cu and -Cu calves, and greater responses at all levels of copper (1 to 16 micrograms/mL) were noted in -Cu calves. These results indicate that copper deficiency affects various physiological characteristics that may be important in immunological defense to pathogenic challenge.

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