J. Anim Sci.
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Journal of Animal Science, Vol 71, Issue 2 467-470, Copyright © 1993 by American Society of Animal Science

JOURNAL ARTICLE

Technical note: an improved method for extraction and quantification of toxic phenethylamines from Acacia berlandieri

I. J. Pemberton, G. R. Smith, T. D. Forbes and C. M. Hensarling
Texas A&M University Agricultural Research and Extension Center, Overton 75684.

N-methyl-beta-phenethylamine (NMPEA) has been previously identified as the toxin causing locomotor ataxia in sheep and goats grazing the browse plant, Acacia berlandieri. We describe a simplified procedure for extraction and quantification of naturally occurring beta-phenethylamines from this Acacia species. Dried, ground plant tissue was extracted (1:20 wt/vol) with 1% glacial acetic acid and filtered. The filtrate was passed through a high-sulfonated polymeric solid-phase extraction (SPE) tube, which retained the compounds of interest (tyramine, hordenine, NMPEA) but allowed many impurities co-extracted from the plant tissue to be washed through. Amines were eluted from the tube, then separated and detected by reversed-phase HPLC. Extracted amines were resolved by HPLC in < 15 min, and UV-absorbance spectra matched those of authentic standards. Recovery efficiency of amine standards (125 micrograms/mL) from SPE tubes averaged 97, 101, and 98% for tyramine, hordenine, and NMPEA, respectively. Excess sample loss was prevented and the large volumes of solvents required for liquid-liquid extraction eliminated by use of solid-phase extraction techniques.




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Copyright © 1993 by the American Society of Animal Science.