J. Anim Sci.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by McMillan, D. N.
Right arrow Articles by Maltin, C. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by McMillan, D. N.
Right arrow Articles by Maltin, C. A.

Journal of Animal Science, Vol 70, Issue 10 3014-3023, Copyright © 1992 by American Society of Animal Science

JOURNAL ARTICLE

The effect of the beta-adrenergic agonist clenbuterol on growth and protein metabolism in rat muscle cell cultures

D. N. McMillan, B. S. Noble and C. A. Maltin
Physiology Division, Rowett Research Institute, Bucksburn, Aberdeen, Scotland.

Cultures were established from neonatal rat muscle cells, satellite cells, and L6 myoblasts and changes in protein metabolism were determined as development proceeded. For all three cell types, culture protein content increased with increasing myotube content. The beta-adrenergic agonist clenbuterol (added to a final concentration of 10(-7) M) significantly stimulated fusion (as indicated by creatine kinase activity) in neonatal muscle cultures and also increased culture protein content. This was associated with a stimulation in both the fractional (ks, percentage/day, +13%, P less than .05) and absolute (As, micrograms/day, +19%, P less than .05) rates of protein synthesis within 24 h after drug administration. At 48 h, As was increased by 42% above that of controls (P less than .01). In contrast, in satellite cell cultures, clenbuterol had no consistent effects on either protein accretion, creatine kinase activity, or protein synthesis (ks and As). Similarly, the drug had no stimulatory effect on protein synthesis and protein accretion in L6 myoblast or L6 myotube cultures (and no effect in neonatally derived fibroblast cultures). It is concluded that the fusion response to clenbuterol and, therefore, changes in protein metabolism and protein accretion are greatly dependent on the origin and genetic integrity of muscle cells.


This article has been cited by other articles:


Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
L. C. C. Navegantes, N. M. Z. Resano, A. M. Baviera, R. H. Migliorini, and I. C. Kettelhut
Effect of sympathetic denervation on the rate of protein synthesis in rat skeletal muscle
Am J Physiol Endocrinol Metab, April 1, 2004; 286(4): E642 - E647.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
A. A. Sneddon, M. I. Delday, and C. A. Maltin
Amelioration of denervation-induced atrophy by clenbuterol is associated with increased PKC-alpha activity
Am J Physiol Endocrinol Metab, July 1, 2000; 279(1): E188 - E195.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
H. F. M. Van Der Heijden, P. N. R Dekhuijzen, H. Folgering, L. A. Ginsel, and C. L. A. Van Herwaarden
Long-term effects of clenbuterol on diaphragm morphology and contractile properties in emphysematous hamsters
J Appl Physiol, July 1, 1998; 85(1): 215 - 222.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1992 by the American Society of Animal Science.