J. Anim Sci.
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J. Anim Sci. 1948. 7:283-290.
© 1948 American Society of Animal Science
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The Freezing Point Depression of Bull Semen and Its Relation to the Diluter Problem

G. W. Salisbury1, C. B. Knodt2 and R. W. Bratton

Cornell University

Abstract

From cryoscopic data it was found that fresh, normal bull semen has an osmotic pressure approaching that of cattle blood. The osmotic pressure of bull semen increased with length of storage in the excurrent ducts of the male or outside the body. The semen of two low fertility bulls depressed the freezing point more than did that of normal, fertile bulls. Heating sodium citrate solutions increases their osmotic pressure. For heated solutions, 2.9 grams of Na3C6H5O7 • 2H2O per 100 ml. of water distilled in glass is isotonic with blood.

Footnotes

1 Now at Department of Dairy Production, University of Illinois, Urbana, Illinois.

2 Now at Department of Dairy Husbandry, Pennsylvania State College, State College, Pennsylvania.






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Copyright © 1948 by the American Society of Animal Science.