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The Effect of Chlorobutanol on Plasma Ascorbic Acid Levels and Semen Production in Sheep¹

State College of Washington

Abstract

The feeding of chlorobutanol to sheep was studied to determine the effect it might have on various constituents of the blood of ewes and rams and on sexual activity and semen production of rams.

The first phase was carried out primarily to determine the proper dosage of chlorobutanol to feed rams. A daily dose of 8 or 10 grams of chlorobutanol produced anesthesia. No harmful effects were observed from feeding 6 grams daily to rams.

In the first experiment when treated and control groups of rams were kept together, no differences were found in blood plasma ascorbic acid levels between groups. In the second experiment when treated and control groups were kept separate a marked increase was observed in the plasma ascorbic acid levels of rams fed 6 grams of chlorobutanol daily.

In ewes being fed 4 grams chlorobutanol daily, there was an immediate increase in plasma ascorbic acid levels.

In this experiment no differences were noted between treated and untreated animals in hemoglobin, or plasma carotene and vitamin A levels.

There was no increase in libido nor any definite improvement of semen production and quality in the chlorobutanol fed rams.

¹ Published as Scientific Paper No. 747, College of Agriculture and Agricultural Experiment Stations, Institute of Agricultural Sciences, State College of Washington, Pullman.