Journal of Animal Science, Vol 69, Issue 8 3241-3250, Copyright © 1991 by American Society of Animal Science

JOURNAL ARTICLE

Isolation and culture of fetal porcine myogenic cells and the effect of insulin, IGF-I, and sera on protein turnover in porcine myotube cultures

J. R. Hembree, M. R. Hathaway and W. R. Dayton
Dept. of Anim. Sci., University of Minnesota, St. Paul 55108.

Porcine myogenic cells isolated from 50 to 55-d porcine fetuses were frozen and stored in liquid nitrogen until they were needed to establish cultures. Approximately 75.8 +/- .59% of the clonal cultures established from these frozen stocks produced myotubes and 60.8 +/- 2.3% of the nuclei in differentiated mass cultures were in myotubes. Differentiated cultures contained higher levels of creatine phosphokinase activity than undifferentiated cultures. Additionally, differentiated cultures incorporated [35S]methionine into putative myosin heavy chain, alpha-actinin, and actin more rapidly than did undifferentiated cultures. Insulin, insulin-like growth factor I, and sera stimulated total protein synthesis rate and decreased total protein degradation rate in myotube cultures. Based on our initial characterization, we believe that we have developed an effective and practical procedure for isolating and culturing fetal porcine myogenic cells.

This article has been cited by other articles:

				G. Xi, M. R. Hathaway, W. R. Dayton, and M. E. White Growth factor messenger ribonucleic acid expression during differentiation of porcine embryonic myogenic cells J Anim Sci, January 1, 2007; 85(1): 143 - 150. [Abstract] [Full Text] [PDF]

				T. G. McDaneld, K. Hannon, and D. E. Moody Ankyrin repeat and SOCS box protein 15 regulates protein synthesis in skeletal muscle Am J Physiol Regulatory Integrative Comp Physiol, June 1, 2006; 290(6): R1672 - R1682. [Abstract] [Full Text] [PDF]

				A. T. Waylan, J. P. Kayser, D. P. Gnad, J. J. Higgins, J. D. Starkey, E. K. Sissom, J. C. Woodworth, and B. J. Johnson Effects of L-carnitine on fetal growth and the IGF system in pigs J Anim Sci, August 1, 2005; 83(8): 1824 - 1831. [Abstract] [Full Text] [PDF]

				G. J. Hausman and S. P. Poulos A method to establish co-cultures of myotubes and preadipocytes from collagenase digested neonatal pig semitendinosus muscles J Anim Sci, May 1, 2005; 83(5): 1010 - 1016. [Abstract] [Full Text] [PDF]

				M S Pampusch, G Xi, E Kamanga-Sollo, K J Loseth, M R Hathaway, W R Dayton, and M E White Production of recombinant porcine IGF-binding protein-5 and its effect on proliferation of porcine embryonic myoblast cultures in the presence and absence of IGF-I and Long-R3-IGF-I J. Endocrinol., April 1, 2005; 185(1): 197 - 206. [Abstract] [Full Text] [PDF]

				C. R. Kerth, J. L. Montgomery, K. J. Morrow, M. L. Galyean, and M. F. Miller Protein turnover and sensory traits of longissimus muscle from implanted and nonimplanted heifers J Anim Sci, July 1, 2003; 81(7): 1728 - 1735. [Abstract] [Full Text] [PDF]

				J. Castillo, P.-Y. Le Bail, G. Paboeuf, I. Navarro, C. Weil, B. Fauconneau, and J. Gutierrez IGF-I binding in primary culture of muscle cells of rainbow trout: changes during in vitro development Am J Physiol Regulatory Integrative Comp Physiol, September 1, 2002; 283(3): R647 - R652. [Abstract] [Full Text] [PDF]