J. Anim Sci.
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Journal of Animal Science, Vol 69, Issue 6 2303-2309, Copyright © 1991 by American Society of Animal Science

JOURNAL ARTICLE

Effect of group thawing on post-thaw viability of bovine spermatozoa packaged in .5-milliliter French straws

D. W. Brown Jr, P. L. Senger and W. C. Becker
Washington State University, Pullman 99164-6332.

The objective of this study was to determine the effects of thawing groups of 2, 5, 10, 15, or 20 .5-ml French straws on post-thaw spermatozoal viability. Thermostatically controlled and nonthermostatically controlled thawing baths were compared. Using a split-plot design, semen from 10 bulls was extended in egg yolk citrate, frozen, and then thawed (in the respective groups) at 36 degrees C in two types of thawing baths. Motility and percentage of intact acrosomes were determined immediately after thawing (0 h) and again after 4 h of incubation at the respective temperature of each thawing bath. Neither percentage of intact acrosomes nor motility was influenced by the number of straws thawed at 0 h (P greater than .05). Thawing bath had no effect (P greater than .05) on motility or percentage of intact acrosomes at 0 h. Bull variation was significant in both the 0- and 4-h evaluations. After 4 h of incubation, there was a significant (P less than .05) straw number x thawing bath interaction. When 15 or 20 straws were thawed in the thermostatically controlled bath there was a reduction (P less than .05) in motility and percentage of intact acrosomes. However, in the nonthermostatically controlled bath there was no reduction in motility and percentage of intact acrosomes as the size of straw group increased. Our results indicate that, when using a nonthermostatically controlled thawing bath, semen packaged in .5-ml straws can be thawed in groups of 20 without an effect on post-thaw sperm viability.


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J. C. Dalton, A. Ahmadzadeh, B. Shafii, W. J. Price, and J. M. DeJarnette
Effect of Simultaneous Thawing of Multiple 0.5-mL Straws of Semen and Sequence of Insemination on Conception Rate in Dairy Cattle
J Dairy Sci, April 1, 2004; 87(4): 972 - 975.
[Abstract] [Full Text] [PDF]


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Copyright © 1991 by the American Society of Animal Science.