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Journal of Animal Science, Vol 68, Issue 10 3360-3370, Copyright © 1990 by American Society of Animal Science

JOURNAL ARTICLE

Quantity and characteristics of microorganisms associated with ruminal fluid or particles

J. A. Olubobokun and W. M. Craig
Dept. of Anim. Sci., Louisiana State University Agricultural Center, Baton Rouge 70803.

An experiment was conducted to 1) determine quantity of microbial CP and DM associated with undigested feed particles, 2) quantify the amount of microorganisms removed from ruminally incubated forages and 3) compare forage disappearances (D) corrected for microbial contamination, using diaminopimelic acid (DAPA) ratios obtained from particle-associated or fluid-associated microorganisms. Samples of alfalfa hay, bermudagrass hay and orchardgrass hay placed in dacron bags were incubated via ruminal cannula for 6 and 12 h. Whole ruminal contents (WRC) were sampled at the time of bag removal and partitioned into fluid- and particle-associated microorganisms. Particle-associated microorganisms were further divided into loosely-associated (LA) and firmly associated (FA) microorganisms. Percentage microbial CP and DAPA, quantities of OM, CP (mg) and DAPA (micrograms), microbial contamination and microbial contribution to ruminal contents were not affected by time (P greater than .05). The highest concentrations of CP and DAPA were found in fluid and LA, respectively (P less than .01). The total amount (content) of OM, CP and DAPA were highest in FA and lowest in microbes in the fluid fraction. Firmly-associated microorganisms constituted 54.1% of the particle-associated population. Corrected DMD were higher (P less than .01) based on FA than on fluid and LA microbe compositions. Corrected CPD were similar (P greater than .10) between populations except at 6 h, when LA corrected CPD were lower. Theoretically, using FA microorganisms should give the most accurate correction for microbial contamination. However, results indicate that, although the three populations differed in composition, fluid and FA corrected CPD were similar. Corrections based on composition of any of these three populations will yield higher estimates of disappearance compared with uncorrected values.


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M. Rodriguez-Prado, S. Calsamiglia, and A. Ferret
Effects of Fiber Content and Particle Size of Forage on the Flow of Microbial Amino Acids from Continuous Culture Fermenters
J Dairy Sci, May 1, 2004; 87(5): 1413 - 1424.
[Abstract] [Full Text] [PDF]


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Copyright © 1990 by the American Society of Animal Science.