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Insulin-Like Growth Factor 1 Induction of Differentiation of Porcine Preadipocytes^1,2,3,4,

The Ohio State University and The Ohio Agricultural Research and Development Center, Columbus 43210

Abstract

The role of insulin-like growth factor I (IGF-1) in the development of the porcine preadipocyte was studied. Primary cultures of stromal-vascular cells (containing preadipocytes) were derived from s.c. adipose tissue of pigs at 1 d of age by enzyme digestion and centrifugation. Cells were cultured for a total of 15 d. Cells were exposed to IGF-1 at concentrations of 0, 5, 25 or 50 ng/ml medium during one of four time periods: d 1–15, d 1–5, d 13–15, or 4 h on d 15 of culture. IGF-1 had a mitogenic effect on cells during the first three time periods as determined by coulter counting. IGF-1 induced the enzymatic differentiation of porcine preadipocytes following exposure for either the entire 15 d of culture or for only 48 h (d 13–15) after confluency had been attained (d 5). Histochemically, lipid accumulation over time paralleled changes in enzyme activity. Incubation of IGF-1 with the cell cultures during the logarithmic phase of growth (d 1–5) or for 4 h on d 15 did not affect enzyme activity. These data indicate that IGF-1 can induce the differentiation of porcine preadipocytes after the cells leave the logarithmic phase of growth through action on post-confluent events.

¹ This research was supported by USDA Gram #87-CRCR-1-2533.

² Salaries and research facilities provided by State and Federal funds appropriated to the Ohio Agric. Res. and Dev. Center, The Ohio State Univ., Journal Article No. 232-88.

³ Dept. of Anim. Sci.

⁴ The authors thank J. Knauerhase for typing the manuscript and M. D. Bishop for statistical analysis.

⁵ To whom reprint requests should be addressed.

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