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The Ohio State University, Wooster 44691 and University of Florida, Gainesville 32611
Abstract
Uterine luminal fluids (ULF) from Large White (LW) and prolific Chinese Meishan (MS) gilts were compared with respect to their peptide growth factor content during an estrous cycle and early pregnancy. Insulin-like growth factor-I (IGF-I) was quantitated by RIA; in vitro growth promoting properties of uterine luminal fluid mitogen (ULFM) were measured by [3H]thymidine incorporation into DNA of quiescent AKR-2B fibroblastic cells in culture. Peak concentrations (pg/µg ULF protein) of IGF-I in ULF of Large White and Meishan gilts, respectively, were: estrous cycle, 9.8 ± 1.4 (on d 10) and 39.7 ± 7.8 (on d 12); gestation, 13.1 ± 3.2 (on d 8 and 10) and 11.9 ± 2.1 (on d 12), with differences among days (except d 10, P > .5) being affected by breed (P < .10). For both breeds, there was a rapid decline in IGF-I concentrations by d 14 of the cycle and of pregnancy. Uterine luminal fluid mitogen activity was greater (P < .01) for LW than for MS gilts on d 10 to 14 of an estrous cycle and gestation and diminished in a time-dependent manner in both breeds. No correlation was observed between IGF-I concentrations and uterine weights for either breed. In contrast, a negative correlation between uterine weight and ULFM activity was detected for cyclic (MS: r = –.855, P < .10; LW: r = –.834, P < .05) and pregnant (MS: r = –.806, P < .10; LW: r = –.928, P < .05) gilts. Immunoreactivity of IGF-I was temporally correlated with rapid and uniform development of conceptuses from MS, but not from LW, gilts. In summary, growth factor content of ULF from LW and MS gilts differed in a quantitative and temporal fashion, but this difference was not sufficient per se to account for variations in reproductive performance.
1 We thank Michel Terqui, Institut National de la Recherche Agronomique, and Francoise Martinat-Botte, Institute Technique du Porc and the USDA Office of Int. Coop. and Dev., for supporting this study. The technical assistance of Cindy Coy and secretarial expertise of Norma Rickett are gratefully acknowledged. This work was supported by the FIRST award (HD 21961) from the Natl. Inst. of Health to R.C.M.S. Salaries and research support were provided also by state and federal funds appropriated to the Ohio Agric. Res. and Dev. Center. This paper is published as OARDC Journal Article no. 104-88.
2 Dept. of Anim. Sci. and Lab. of Mol. and Devel. Biol., Ohio State Univ.-OARDC.
4 Dept. of Anim. Sci., Univ. of Florida.
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