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University of Nebraska,4 Lincoln 68583-0908
Abstract
Three strains of Lactobacilus acidophilus (LA) were isolated from the feces of mature boars that were not being fed antibiotics from the Nebraska Gene Pool (NGP). All three LA isolates were screened in vitro for anticholesteremic and antimicrobial activities. One strain, LA16, caused the greatest reduction in cholesterol and inhibited both Bacillus subtilis and Escherichia coli the most. LA16 was used to produce 16, 18.9-liter quantities of acidophilus yogurt (AY), over a period of 8 wk, for use as a feed ingredient in diets for the NGP boars. Colony forming units (cfu), pH, protein, energy, Ca and P were consistent across all 16 batches of yogurt. All of the 18 boars were fed a high-cholesterol diet for a period of 56 d at a rate of 2.268 kg/(h·d) to furnish 6.661 g/(h·d) of cholesterol. Nine of the boars then were fed 1.81 kg/(h·d) of a second diet that was supplemented with .454 kg/(h·d) of AY. The other nine boars were fed the original diet. Cholesterol intake was the same for the two dietary treatments. Blood samples were collected weekly from the brachial-jugular region and the sera were analyzed for lipids. Acidophilus yogurt reduced serum cholesterol (P < .01) and low density lipoproteins (P < .08), but it had no effect on serum triglycerides (P > .23) or on high density lipoproteins (P > .11).
1 Published as paper no 8543, Journal Series Nebraska Agric. Res. Div.
2 The authors thank Henningsen Foods Inc. (Dwight Bergquist) and the Dairy Bureau of Canada for contributing products and partial financial support for this project.
3 Technical assistance of Sandra Frank, Diana J. Smith and Roy L. Carlson was appreciated.
4 Dept. of Anim. Sci. Reprint requests should be sent to E. R. Peo, Jr.
5 Dept. of Food Sci. and Technol.
6 Dept. of Food Sci. and Agric. Chem., McGill Univ., Montreal Quebec, Can.
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