J. Anim Sci.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J. Anim Sci. 1989. 67:1082-1089.
© 1989 American Society of Animal Science
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Stone, R. T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Stone, R. T.

Isolation and Characterization of Recombinant cDNA Clones Corresponding to Developmentally Regulated Genes in Pig Liver

R. T. Stone1,2,

U.S. Department of Agriculture3, Clay Center, NE 68933

Abstract

To identify clones that corresponded to developmentally regulated genes in pig liver, recombinant cDNA libraries were constructed from early fetal (d 40), late fetal (d 110) and adult liver mRNA. Each library was screened by probing with total cDNA prepared from mRNA at different stages of development. Nine clones that gave distinct developmental patterns when hybridized to liver RNA isolated from various stages of development were subsequently isolated and partially sequenced. The cognate proteins for seven of these clones were identified by searching a national DNA sequence resource (Bionet) for similar sequences. Clones hybridizing to mRNA that was most abundant early in development were {alpha} fetoprotein, at {alpha}1 antiprotease, {alpha} globin and an unidentified clone. The mRNA for ß and {gamma} fibrinogen were most abundant perinatally. The abundance of mRNA for albumin, haptoglobin and a second unidentified clone was low in fetal liver, but increased to adult levels between birth and 3 d of age. These clones provide probes to study the relationship between factors affecting fetal growth and expression of specific genes throughout the development.

Footnotes

1 ARS, Roman L. Hruska U.S. Meat Anim. Res. Center, P. O. Box 166, Clay Center, NE 68933.

2 The author acknowledges the technical assistance of Misi Robinson.

3 Mention of a trade name, proprietary product or specific equipment does not constitute a guarantee or warranty by the USDA and does not imply approval to the exclusion of other products that may be suitable.






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1989 by the American Society of Animal Science.