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University of Alberta,4, Edmonton, Alberta T6G 2P5
Abstract
Barrows with an average initial weight of 55 kg were fitted with simple T-cannulas at the distal ileum. The animals were fed a protein-free diet that consisted of 79.7% cornstarch, 10% sucrose, 3% Alphafloc (a source of cellulose), 3% canola oil and a vitamin-mineral premix. The pigs were fed 700 g of diet twice each day, at 0800 and 2000. A balanced amino acid mixture or a saline solution was administered intravenously while the protein-free diet was fed. Heal digesta were collected for 24 h following a 7-d adaptation period. The administration of amino acids reduced (P < .05) the recovery of endogenous protein from 18.5 to 12.7 g per kg dry matter intake. For the amino acids, the reduction was only significant (P < .05) for proline, from 3.6 to .6 g per kg dry matter intake. If the total endogenous protein losses are assumed to be constant and the differences in the amino acid composition of non-reabsorbed endogenous protein, as observed in this study, are used to calculate true ileal digestibilities, differences in the digestibilities of the indispensable amino acids are large (up to 7.4 percentage units for threonine). The amino acid composition of endogenous protein determined in pigs fed a protein-free diet and parenterally administered with amino acids should provide a better estimate for the calculation of true amino acid digestibilities when based on the determination of true protein digestibility by the 15N-isotope dilution technique.
1 Financial support was provided by the Fanning for the Future Program of the Alberta Agric. Res. Council. The authors acknowledge the assistance of Brenda Kozak and Charlene Shellian and the excellent preparation of this manuscript by Jan Oldfield. Series No. 10535.
2 To whom requests for reprints should be addressed.
3 Academy of Agric. Sci., Oskar Kellner Institute, Rostock, G.D.R.
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