J. Anim Sci.
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J. Anim Sci. 1989. 67:432-440.
© 1989 American Society of Animal Science
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The Evaluation of the Mobile Nylon Bag Technique for Determining the Apparent Protein Digestibility in a Wide Variety of Feedstuffs for Pigs

W. C. Sauer1, L. A. den Hartog2, J. Huisman3, P. van Leeuwen3 and C.F.M. de Lange1

Agricultural University of The Netherlands,4, Wageningen, The Netherlands

Abstract

Studies were conducted with four (50 to 70 kg) barrows to evaluate the mobile nylon bag technique (MNBT) for determining the apparent protein digestibility (AD) in a variety of feedstuffs. One-gram samples of each feedstuff, ground through a 1.0-mm mesh screen, were enclosed in nylon bags (25 x 40 mm; 48 µm mesh). Following predigestion (.01 N HCl; 4,000 Folin Intestinal Pepsin Units/liter), four bags of each sample were inserted into each pig via a duodenal T-cannula. The indigested contents were pooled within pig and feedstuff prior to crude protein analysis. The AD of each feedstuff was determined previously with four barrows according to the conventional method (CM), based on a 5-d total collection of feces following a 14-d adaptation period. The following results, AD (%), were obtained with the MNBT (n = 4) and CM (n = 4), respectively. Barley, 70.1 vs 81.2 (P < .05); corn, 72.3 vs 82.1 (P < .05); wheat, 79.6 vs 86.4 (P < .05); corn gluten feed, 73.9 vs 70.9; rice bran, 57.3 vs 57.4; wheat middlings, 70.8 vs 79.4 (P < .05); soybean meal, 89.9 vs 93.0; a mixture of soybean meal and hulls, 86.9 vs 88.8; coconut expeller, 65.0 vs 69.8; fababeans, 79.5 vs 78.5; peas, 87.6 vs 88.1; meat meal ,83.2 vs 84.0; complete diet (4.3% crude fiber), 76.3 vs 83.6 (P < .05); and complete diet (13.6% crude fiber), 56.5 vs 55.0. Although the AD in the cereals, wheat middlings and the complete diet with 4.3% crude fiber were lower (P < .05) by the MNBT than by the CM, a correlation of .925 was established between both methods by simple regression analysis. An improvement (P < .05) in the correlation coefficient to r = .949 was obtained when the N-free extract or crude fiber content in the feedstuff was included as a second covariable in multiple regression analysis.

Footnotes

1 Dept. of Anim. Sci., Univ. of Alberta, Edmonton, Canada T6G 2P5; to whom requests for reprints should be addressed.

2 Dept. of Anim. Nutr. Agric. Univ. of the Netherlands, Wageningen, The Netherlands.

3 Res. Inst. of Anim. Nutr. and Toxicol. (ILOB/TNO), Wageningen, The Netherlands.

4 Appreciation is expressed to D. van Kleef and K. Deuring for their technical assistance.






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Copyright © 1989 by the American Society of Animal Science.