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Rapid [¹⁴C] Heptachlor Clearance from Body Stores of Ovines: Ingested Mineral Oil and Parenteral Trans-Stilbene Oxide Lack Effects¹

New Mexico State University, Las Cruces 88003; Kansas University Medical Center, Kansas City 66103; and Institut fur Toxikologie, der GSF Munchen mbH, 8042-Neuherberg, Federal Republic of Germany

Abstract

Recently we reported elimination of radioactivity from [¹⁴C] heptachlor from body stores of lactating ovines, mainly into excreta rather than milk, contrasting sharply with bovines. To further assess heptachlor metabolism and clearance by ovines, 12 fine-wool wether lambs (41 ± 3 kg) housed in metabolism stalls were fed pelleted alfalfa hay (96%) plus molasses (3%) ad libitum and were dosed i.p. once with [¹⁴C] heptachlor (1.643 mg/kg body wt; sp. act. = .89 µCi/mg). Feces and urine were collected separately and quantitatively. Light mineral oil was mixed with feed (5 g/100 g) of six lambs and trans-stilbene oxide, an inducer of biotransformational enzymes, was administered i.p. (4 g/hd initially; 2 g/hd daily thereafter) through 20 d to three lambs given each mineral oil treatment, in 2 x 2 factorial arrangement. Feces, urine, blood, bile and body tissues were assayed for total ¹⁴C activity. Radioactivity (heptachlor and [or] metabolites) eliminated into excreta during 21 d amounted to 34 to 36% of dose administered, of which 67% appeared in urine and 33% in feces. Biological half-time for elimination into excreta was 11.3 d [K_el = –.061/d], similar to 11.7 d we reported for lactating ewes. Clearance from blood had T = 14 d. Neither mineral oil nor trans-stilbene oxide altered rate or route of ¹⁴C activity excreted or concentrations of ¹⁴C activity in blood. Results confirmed that ovines eliminate heptachlor much more rapidly than bovines.

¹ Journal article 1389 of the New Mexico Agric. Exp. Sta., Las Cruces.

² Dept. of Anim. and Range Sci., N. M. State Univ. Direct reprint requests to G. S. Smith.

³ Dept. of Pharmacol., Toxicol, and Therapeutics, Kansas Univ. Med. Ctr. Technical assistance of Margita Hoefler is acknowledged.

⁴ Inst. fur Toxikol., GSF provided materials and support.