J. Anim Sci.
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J. Anim Sci. 1988. 66:2401-2406.
© 1988 American Society of Animal Science
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Suppressed Development of Cultured Mouse and Swine Embryos by Diethylstilbestrol1

C. S. Gardiner, A. R. Menino, Jr., A. E. Archibong, J. S. Williams, F. Stormshak and D. C. England

Oregon State University2, Corvallis 97331-6702

Abstract

Effects of prolonged exposure to the synthetic estrogen diethylstilbestrol (DES) on in vitro development of early mouse and swine embryos were investigated. Two-cell mouse embryos cultured in Whitten's medium (WM) for 192 h were exposed to 10–4, 10–7 or 10–10 M DES dissolved in 1, 10–3 or 10–6 % ethanol, respectively. One-cell to eight-cell swine embryos were cultured in WM for 192 h containing 10–4 or 10–7 M DES dissolved in 1 and 10–3 % ethanol, respectively. Embryos cultured in WM containing 1 (0 DES,), 10–3 (0 DES2) or 10–6 % ethanol (0 DES3) served as controls. Hatching was inhibited (P < .05) in mouse embryos cultured in 10–4 M DES (3.0 ± 2.1% vs 0 DES1, 25.1 ± 3.7%). Similar (P > .10) percentages of mouse embryos hatched in 10–7 M DES (36.4 ± 5.4% vs 0 DES2, 29.1 ± 5.7%) and 10–10 M DES (44.4 ± 4.4% vs 0 DES3, 38.9 ± 5.3%). Diethylstilbestrol at a concentration of 10–4 M failed to affect the development of one- to eight-cell swine embryos into blastocysts. However, compared with 0 DES2, 10–7 M DES reduced (P < .05) the number of swine blastocysts developing from one- to two-cell (36 vs 78%) and three- to four-cell embryos (50 vs 84%). No significant effects of 10–7 M DES were detected on the ability of six- to eight-cell swine embryos to develop into blastocysts. The results suggest that the detrimental effects observed on blastocyst formation in swine and on hatching in the mouse vary with concentration of DES and embryonic stage of development at onset of treatment.

Footnotes

1 Tech. paper no. 8412, Oregon Agric. Exp. Sta.

2 Dept. of Anim. Sci.






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Copyright © 1988 by the American Society of Animal Science.