J. Anim Sci.
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J. Anim Sci. 1988. 66:947-953.
© 1988 American Society of Animal Science
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Co-Culture of Ovine Ova with Oviductal Cells in Medium 199

C. E. Rexroad, Jr. and A. M. Powell1,2,3

U.S. Department of Agriculture, Beltsville, MD 20705

Abstract

Three experiments were conducted to test the suitability of medium 199 supplemented with 10% fetal calf serum (M199FCS) as a medium for co-culture of one-cell sheep ova with sheep oviductal cells. In Exp. 1, ova were co-cultured for 5 d in 5 ml of M199FCS or in Ham's F10 medium supplemented with 10% fetal calf serum (F10FCS). Co-culture did not increase the number of cleavages at the end of 5 d of culture, but M199FCS supported more cleavages than did F10FCS (P = .016). In Exp. 2, ova were cultured for 1 to 3 d in M199FCS alone or on oviductal, uterine or kidney cell monolayers from ewes 2 d postestrus and transferred to recipients from which they were recovered at 8 d postestrus. Co-culture with oviductal cells improved (P < .001) the cleavage index of recovered embryos compared with culture in medium alone or co-culture with other cell types. In Exp. 3, monolayers of oviductal cells from ewes 2 d postestrus and from luteal-phase ewes were cultured as in Exp. 2. No difference was observed between the two sources of oviductal cells for their ability to support in vitro development of one-cell sheep eggs for 1 or 2 d. These studies suggest thatM199FCS may be a good medium to use in an oviductal cell co-culture system for one-cell sheep ova. Results further suggest that specific secretions of oviductal cells may be important for early embryo development in vivo.

Footnotes

1 Agric. Res. Serv., Beltsville Agric. Res. Center, Ani. Sci. Inst., Reprod. Lab.

2 The authors thank Linda Neuenhahn for excellent manuscript preparation.

3 Mention of companies or products in this report does not constitute endorsement by the USDA to the exclusion of others not mentioned.






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Copyright © 1988 by the American Society of Animal Science.