J. Anim Sci.
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J. Anim Sci. 1988. 66:845-850.
© 1988 American Society of Animal Science

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Visual and Chemical Tissue Markers for Bovine Carcass Components1

R. Y. Lary2, F. M. Byers3, H. R. Cross2, G. T. Schelling3 and H. D. Petersen4

Texas A&M University and Texas Agricultural Experiment Station, College Station 77843

Abstract

A two-component, nontoxic, quantifiable animal/carcass tracing system was developed using riboflavin as an on-premises, initial carcass identifier visible under longwave ultraviolet (UV) light and deuterium oxide (D2 O) as a tracer analytically quantified via fixed wavelength infrared spectrophotometry. Twenty-four cull cows and heifers were allocated into eight antemortem treatment groups (1, 12, 24, 48, 72, 96, 120, 144 h) for evaluation of the efficacy of riboflavin and D2 O as tissue tracers in postmortem meat tissues. All cattle were slaughtered using conventional procedures and inspection. To study postmortem riboflavin marker changes due to constant light exposure over time, fluorescence and emission intensity scores were obtained by a trained panel 24, 48, and 168 h postslaughter. The riboflavin marker intensity rating means for UV fluorescence were classified as identifiable on all carcasses when evaluated under UV light, but were classified as not identifiable when evaluated under ambient light. Deuterium oxide levels in all tissue water samples, regardless of antemortem infusion group, contained D2 O concentrations at least 2.5 times greater than those found in background water. Deuterium oxide was shown to disperse rapidly throughout living tissues. Correlations within animals for D2 O levels from blood and muscle were all highly significant (r = .99).


Footnotes

1 Journal article TA 22-871, Texas Agric. Exp. Sta. This project was supported in part through funding from FSIS, USDA, Washington, DC.

2 Meats and Muscle Biol. Section, Dept. of Anim. Sci.

3 Beef Cattle Nutrition and Growth Section, Dept. of Anim. Sci.

4 USDA, ARS, Texas A&M.







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Copyright © 1988 by the American Society of Animal Science.