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Effect of Zearalenone on Days 7 to 10 Postmating on Intrauterine Environment and Migration of Embryos in Sows^1,2,

Purdue University, West Lafayette, IN 47907

Abstract

On d 7 to 10 postmating, first-litter sows were fed either a control diet or a diet containing zearalenone (ZEN; 1 mg/kg body weight). Surgery was performed on either d 9, 11 or 13 postmating to collect blastocysts and uterine flushings. The rostral and caudal portion of each uterine horn was flushed with phosphate buffered saline, and the blastocysts were separated from the recovered solution. Uterine flushings were analyzed for total Ca, Mg, Zn, estradiol-17ß (E₂ 17ß) and progesterone (P₄). Administration of ZEN did not affect the number of blastocysts recovered or the position of embryos within the uterus on d 9 or 11. Blastocysts recovered on d 13 were filamentous and could not be enumerated. Total Ca in uterine flushings of control sows was higher (P<.001) on d 11 than on d 9 or 13, but intrauterine Ca of ZEN-treated sows did not vary by sampling day (P>.05) and was lower (P=.01) than that of controls on d 11. Total intrauterine Mg of ZEN sows was greater (P=.002) than of control sows on d 11 and 13, and total intrauterine Zn of ZEN sows was greater than that in control sows on d 13. There were no differences in total intrauterine P₄ or E₂ 17ß among ZEN-treated and control sows on d 9, 11 or 13 postmating. Serum concentrations of 13, 14-dihydro-15-ketoprostaglandin F₂ (PGFM) increased from d 9 to 13 in control and ZEN-treated sows, but there were no differences between treatment groups. These data indicate that treatment with ZEN on d 7 to 10 postmating did not alter spacing of blastocysts or inhibit E₂ 17ß secretion by blastocysts through d 11 postmating. Intrauterine environment in ZEN-treated sows was altered on d 11 and 13 postmating, as evidenced by changes in concentrations of intrauterine cations, but no changes were detected in P₄ or E₂ 17ß.

¹ Journal paper no. 11,131, Purdue Univ. Agric. Exp. Sta.

² Presented in part at the 19th Meeting of the Midwestern Section of the Am. Soc. of Anim. Sci., Des Moines, IA, Abstr. No. 124. The authors gratefully acknowledge G. D. Niswender, Colorado State Univ., for supplying antiserum to progesterone; N. R. Mason, Eli Lilly and Co., Indianapolis, IN for providing antiserum to estradiol-17ß; K. T. Kirton, Upjohn Co., Kalamazoo, MI for providing antiserum to PGFM. Purified zearalenone was generously provided by International Mineral and Chemical Corp., Terre Haute, IN. The technical assistance of S. Albregts, D. Blair and K. Knox is greatly appreciated.

³ Present address: Eli Lilly and Co., Greenfield, IN 46140.

⁴ Dept. of Anim. Sci.; Address reprints to this author.

⁵ Dept. of Large Anim. Clinics.