J. Anim Sci.
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J. Anim Sci. 1988. 66:447-451.
© 1988 American Society of Animal Science
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Feedback of 17ß-Estradiol on Secretion of Luteinizing Hormone during Different Seasons of the Year1

T. T. Stumpf, M. L. Day2, P. L. Wolfe, M. W. Wolfe, A. C. Clutter3, R. J. Kittok and J. E. Kinder4

University of Nebraska,5 Lincoln 68583-0908

Abstract

The working hypothesis was that the amount of increase in secretion of luteinizing hormone (LH) that results from positive feedback of 17ß-estradiol (E2) is dependent on season of the year in mature bovine females. Seven beef cows, ovariectomized approximately 2 mo before the initiation of the experiment, were used in the initial year (1983) of the study. Three of the ovariectomized cows (OVX-E2) received an sc E2 implant, which provided low circulating levels of E2. The remaining four cows (OVX) were not implanted. Blood samples were collected serially (at 10-min intervals for 6 h) at each spring and fall equinox and at each summer and winter solstice. This protocol was replicated with a different group of cows in 1985 (OVX-E2, n = 4; OVX, n = 6). Concentration of LH in blood serum was quantified in all samples. Concentration of E2 in blood serum was measured in pools of samples from each serial blood collection. Concentrations of E2 were higher (P<.05) in the implanted cows. Mean concentration of LH and amplitude of pulses of LH were higher (P<.05) at each season of the year in cows that were ovariectomized and implanted with E2 than in cows that were ovariectomized and did not receive E2. An effect of season of the year on mean concentration of LH was detected (P<.01). No influence of season or E2 was detected for frequency of pulses of LH. There was no significant treatmentxseason interaction. Thus, E2 appears to increase the concentration of LH to the same degree during all seasons of the year in the mature bovine female; therefore, our working hypothesis was rejected.

Footnotes

1 Published as paper no. 8 304, Journal Ser. Nebraska Agric. Res. Div. The authors thank K. Hannah for assistance in preparation of the manuscript and Kenneth A. Pearson for technical assistance with hormone analysis. We also thank Jerry J. Reeves for the LH antisera; Norman Mason for the estradiol antisera; and Leo E. Reichert for the purified LH used for iodination.

2 Present address: Dept. of Anim. Sci., The Ohio State Univ., Columbus, OH.

3 Present address: Dept. of Anim. Sci., Oklahoma State Univ. Stillwater, OK.

4 Reprint requests: Dr. James E. Kinder, A224j Anim. Sci.

5 Dept. of Anim. Sci.






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Copyright © 1988 by the American Society of Animal Science.