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Ovine Uterine Morphogenesis: Effects of Age and Progestin Administration and Withdrawal on Neonatal Endometrial Development and DNA Synthesis^1,2,

Auburn University, AL 36849

Abstract

To determine effects of age and administration and withdrawal of a synthetic progestin (P) on endometrial development and DNA synthesis; ewe lambs were ovariectomized on d 0 (birth) and assigned to one of four groups (n = three/group) that provided (by means of hemihysterectomy) the following uterine tissue types: 1) d 0 control, 2) d 13 control, 3) d 26 control, 4) d 13 after 13 d exposure to P (13P) and 5) d 26 after P exposure from d 0 to 13 (26P). Uterine tissues were processed for histology or explanted with [methyl-³ H] thymidine for autoradiography. Labeling index (LI) was determined for stroma and epithelium in caruncular and intercaruncular endometrial areas and for lumenal and glandular epithelium in uteri with glands. Endometrial glands were absent on d 0, evident at d 13 and well developed by d 26. Day 0 LI was greater (P < .05) in caruncular than in intercaruncular areas, and greater in stromal than in epithelial tissues. Relationships were reversed in d 13 endometrium (day x endometrial area, P < .07). Caruncular stromal LI was greater on d 0 than later (P < .02), whereas intercaruncular epithelial LI was greater after d 0 (P < .05), but decreased from d 13 to 26 (P < .05). Glandular epithelial LI was higher on d 13 than on d 26 (P < .03). Administration of P inhibited endometrial gland development and suppressed d 13P intercaruncular LI (P < .05). Withdrawal of P was followed by endometrial gland development and increased (P < .01) intercaruncular epithelial LI in d 26P uteri. Ovary-independent initiation of endometrial gland development involves age- and region-specific alterations in DNA synthesis and could involve negative control.

¹ Alabama Agric. Exp. Sta. Journal Ser. No. 4-881578P.

² This work partially supported by the Alabama NSF/EPSCoR Program in Molecular, Cellular and Developmental Biology (Grant #RII-8610669).

³ Dept. of Anim. and Dairy Sci. and Alabama Agric. Exp. Sta.

⁴ To whom reprint requests should be addressed.

⁵ Dept. of Large Anim. Surgery and Med.

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