J. Anim Sci.
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J. Anim Sci. 1988. 66:2614-2625.
© 1988 American Society of Animal Science
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Effects of Zeranol on In Vitro Growth Hormone Release by Lamb and Rat Pituitary Cells1

C. J. Phelps3, J. P. Wiggins2 and P. J. Wangsness2

The Pennsylvania State University, University Park 16802

Abstract

A series of experiments was conducted to evaluate the effect of zeranol on release and synthesis of growth hormone (GH) by anterior pituitary cells established in either static or continuous flow cultures. Young adult male rats, slaughter-age lambs and juvenile lambs were used as sources of pituitary cells. In static primary cell cultures, no consistent effect of zeranol at 10–7, 10–9 or 10–11 M was demonstrated by either rat or ovine cells. Rat pituitaries established in perifusion culture chambers showed no repeatable response to zeranol. Dissociated cells from lambs established in perifusion culture, however, had significant increases in release of GH in response to 37% of zeranol pulse exposures. When dissociated cells from juvenile lamb pituitaries were used, up to 10-fold increases in GH release consistently were measured within minutes of exposure to zeranol.

Footnotes

1 Approved by the Director of the Pennsylvania Agric. Exp. Sta. as paper No. 7615 in the Journal Series. The authors would like to thank J. Burdette for her excellent technical assistance. International Minerals Corp. for partial support of this work, and Kim A. Gesell for typing the manuscript. Use of the laboratory facilities of W. C. Hymer was greatly appreciated.

2 Dept. of Dairy and Anim. Sci.

3 Dept. of Mol. and Cell. Biol. Present address: Dept. Neurobiol. and Anat., Univ. of Rochester Med. Ctr., Rochester, NY 14642.

4 Hilltop Laboratories, Scottdale, PA.






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Copyright © 1988 by the American Society of Animal Science.