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Production of Ovine Chimeras by Inner Cell Mass Transplantation¹

University of California, Davis 95616

⁴ To whom reprint requests should be addressed.

Abstract

Ovine chimeras were produced by micro-injection of isolated inner cell masses (ICM) into recipient blastocysts. Inner cell masses were isolated by immunosurgery. A total of 57 chimeric embryos was produced, 52 of which were transferred to recipient ewes. Thirty-seven live lambs were born, of which 15 were determined to be chimeric on the basis of blood type analysis. One lamb, although not a hlood chimera, exhibited overt signs of chimerism. An additional six lambs were determined to have developed solely from the injected ICM. The rate of chimerism in live lambs was 43% (16/37) while the survival rate of injected ICM was 59% (22/37). The method presented allows the production of relatively large proportion of viable, chimeric embryos without the use of an intermediate recipient.

¹ The authors thank Drs. J. Rowe and S. Skirrow for their assistance with surgeries and D. Anderson, S. Donahue, M. Dunbar, and L. Millon for their excellent technical assistance.

² Dept. of Anim. Sci., College of Agr. and Environ. Sci.

³ Present address: LHRRB, Harvard Med. School, 45 Shattuck St., Boston, MA 02115.

⁵ Dept. of Reprod., School of Vet. Med.

⁶ Serol. Lab., School of Vet. Med.