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Influence of Picolinic Acid on the Uptake of ⁶⁵Zinc-Amino Acid Complexes by the Everted Rat Gut^1,2,3,

University of Nebraska⁶, Lincoln 68583-0908

⁵ To whom reprint requests should be addressed.

Abstract

Three hundred fifty rats were used in three experiments to: 1) validate the everted gut procedure as an in vitro technique for estimating Zn absorption, 2) determine the effect of increasing ratios of picolinic acid (PA) to Zn on Zn absorption and 3) determine the effect of PA on absorption of Zn and amino acid complexes at pH 6, 7 and 8. In the first experiment the time delay between tissue collection with subsequent storage in ice-cold saline and start of tissue incubation was 0, 10, 20 or 30 min. A linear decrease was observed for ⁶⁵Zn uptake with increasing delay time. Lysine absorption was not affected by delay time. In the second experiment, molar ratios of PA:Zn of 0, .5, 1.0, 1.5, 2.0 and 2.5 with Zn held constant were evaluated. A linear decrease in ⁶⁵Zn absorption from ⁶⁵ZnCl₂ occurred as the molar ratio of PA to Zn increased. In the third experiment, 0 and 5 molar ratios of PA to a constant Zn level were evaluated using ⁶⁵ZnCl₂, ⁶⁵Zn-¹⁴C-methionine (ZnMet) and ⁶⁵Zn-³H-lysine (ZnLys) at pH 6, 7 and 8. The addition of PA decreased Zn absorption regardless of Zn source. The data suggest that the Zn sources used were of similar biological value. The data do not support the theory that PA facilitates Zn absorption.

¹ Published as paper no. 8212, Journal Series, Nebraska Agr. Res. Div.

² Radio-labeled zinc-amino acid complexes donated by ZINPRO Corp., Chaska, MN 55318.

³ The technical assistance of R. M. Hill, Dept. of Agr. Biochem. and Joy Kovar, Dept. of Anim. Sci. is gratefully acknowledged; appreciation is expressed to Diana J. Smith for manuscript preparation.

⁴ Current address: Roman L. Hruska USMARC, Clay Center, NE 68933.

⁶ Dept. of Anim. Sci.