J. Anim Sci.
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J. Anim Sci. 1987. 64:1031-1037.
© 1987 American Society of Animal Science
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Hormonal Control of Porcine Adipose Tissue Fatty Acid Release and Cyclic AMP Concentration1

C. Y. Hu2,3,, J. Novakofski2 and H. J. Mersmann3,4,

University of Illinois2, Urbana 61801 and U. S. Department of Agriculture,3,5,6,, Clay Center, NE 68933

Abstract

Compared with adipose tissue from other mammals, porcine adipose tissue has stringent specificity for stimulation of lipolysis by analogs of norepinephrine. This study was to ascertain whether the specificity for control was reflected in the concentration of tissue cyclic AMP (cAMP). Adipose tissue slices were incubated and concentrations of tissue cAMP and free fatty acids (FA) released to the medium were measured. It was necessary to include theophylline in the incubation medium to be able to measure changes in cAMP concentration. Fatty acid release and cAMP production were increased most effectively by the β-adrenergic agonists: isoproterenol, fenoterol, dobutamine and the mixed {alpha}- + β-adrenergic agonist, epinephrine. Isoproterenol-stimulated FA and cAMP production were both inhibited by the β-adrenergic antagonist, propranolol. There was no evidence for {alpha}2-adrenergic inhibition of lipolysis in porcine adipose tissue because clonidine ({alpha}2-adrenergic agonist) did not lower isoproterenol-induced FA or cAMP levels and phentolamine, an {alpha}-adrenergic antagonist, did not increase epinephrine-stimulated FA release or cAMP generation. These results imply that the stringent specificity observed for stimulation of swine adipose tissue lipolysis resides in the β-adrenergic receptor coupled to cAMP production.

Footnotes

1 A preliminary report of this data was presented in 1985 (Fed. Proc. 44:248, abstr. 802).

2 Dept. of Anim. Sci. Dr. Hu was located at Clay Center, NE. His present address: Dept. of Anim. Sci., Oregon State Univ., Corvallis, OR 97331.

3 Roman L. Hruska, U. S. Meat Anim. Res. Center, ARS, Clay Center, NE 68933.

4 Author to whom reprint requests should be addressed.

5 The authors thank J. A. Dague for biopsy of pigs, M. M. Bierman and C. L. Withrow for secretarial assistance and Dr. M. D. MacNeil for statistical consultation.

6 Mention of trade names, proprietary products or specific equipment does not constitute a guarantee or warranty of the product by the USDA and does not imply its approval to the exclusion of other products that may also be suitable.






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Copyright © 1987 by the American Society of Animal Science.