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A Method for Chronically Quantifying Net Absorption of Nutrients and Gut Metabolites into Hepatic Portal Vein in Conscious Swine^1,2,

Roman L. Hruska U.S. Meat Animal Research Center, Clay Center, NE 68933

Abstract

Surgical procedures are described for chronic cannulation of portal vein, ileal vein, abdominal aorta, and carotid artery in pigs. Silastic or Micro-Renathane tubing was used for cannulating portal vein and ileal vein, while carotid artery was cannulated with Micro-Renathane tubing. The lumen of Micro-Renathane tubing was coated with tri-dodecylmethyl ammonium chloride (TDMAC)-heparin complex. The abdominal aorta was cannulated via saphenous artery with vinyl tubing. This allows simultaneous collection of blood samples from hepatic portal vein and systemic artery (carotid or abdominal aorta) and continuous infusion of p-aminohippuric acid (PAH) into ileal vein. The constant PAH infusion provided an indicator-dilution method for estimating the blood flow rate in portal vein. In 13 pigs weighing 54 ± 2.8 kg, the mean portal vein blood flow rate during the 8-h postprandial period was estimated to be 1,979 ml·min^–1 ·pig^–1 or 37.8 ml·min^–1 ·kg^–1 body weight. By simultaneously measuring the concentration of nutrients and metabolites in the portal and systemic arterial blood and multiplying porto-arterial differences by the estimated portal vein blood flow rate, the net absorption of nutrients (except long-chain fatty acids) and metabolites into hepatic portal system in conscious swine can be quantified.

¹ The authors thank Dr. P. J. Dziuk for the demonstration and suggestions of cannulating the portal vein; Dr. G. B. Huntington for the suggestion and R. V. Mlejnek and Bob Lee for the set-up and for analyzing plasma aminohippuric acid; Dr. L. P. Reynolds for the suggestion of cannulating saphenous artery; Dr. J. J. Ford for the suggestion of using Micro-Renathane tubing; Dr. W. G. Pond and Dr. A. H. Jensen for their interest in the research; Leo la Lansford, Sherrie Prager, Janell Dague, Ted Acton, Suzy Reece, Lynn Laaker, Bruce Larson, Mike Wallace and associates for animal training, management and surgical assistance; Lei Hwa Yen for laboratory expertise; Dr. Mike MacNeil, Jim Wray, Ming Hu and associates for data analysis; and Sherry Hansen for typing the manuscript.

² Mention of trade name, proprietary product, or specific equipment does not constitute a guarantee or warranty by the USDA and does not imply approval to the exclusion of other products that may be suitable.

³ USDA, ARS.

⁴ Univ. of Nebraska, Roman L. Hruska U.S. Meat Animal Research Center, Clay Center, NE 68933.