J. Anim Sci.
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J. Anim Sci. 1987. 64:633-641.
© 1987 American Society of Animal Science
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The Relationship Between Cell Proliferation and Phosphatidylinositol Metabolism in Skeletal Muscle Cells in Culture

Mohammad Koohmaraie1,2,

U.S. Department of Agriculture3, Clay Center, NE 68933

Abstract

Effects of {gamma}-hexachlorocyclohexane (1 x 10–4 M) and lithium chloride (10 mM) on the cell proliferation and phosphatidylinositol metabolism of L6 myoblasts were examined. {gamma}-Hexachlorocyclohexane (inhibitor of phosphatidylinositol synthesis) and LiCl (inhibitor of inositol 1-phosphatase) treatment of these cells resulted in the loss of their proliferative properties. Coordinate with effects on cell proliferation, {gamma}-hexachlorocyclohexane and LiCl also reduced the levels of phosphatidylinositols and their water-soluble metabolites. However, the protein content of treated cells was higher than control cells. Gamma-hexachlorocyclohexane and LiCl treatment of cells reduced DNA synthesis by about 50% over control values. It was, therefore, concluded that there appears to be a direct relationship between L6 myoblast proliferation and phosphatidylinositol metabolism.

Footnotes

1 Present address: Dept. of Food Sci. and Human Nutr., Michigan State Univ., East Lansing, MI 48824.

2 Acknowledgement is given to Dr. J. E. Schollmeyer for technical assistance and critical review of this manuscript. Dr. H. J. Mersmann for helpful discussions and critical review of the manuscript, Dr. M. D. MacNeil for assistance in the statistical analysis of the data and Ms. Jackie Byrkit for secretarial assistance.

3 Mention of trade name, proprietary products or specific equipment does not constitute a guarantee or warranty of the product by the USDA and does not imply its approval to the exclusion of other products that may also be suitable.






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Copyright © 1987 by the American Society of Animal Science.