J. Anim Sci.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J. Anim Sci. 1987. 64:615-622.
© 1987 American Society of Animal Science
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kotts, C. E.
Right arrow Articles by Dayton, W. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kotts, C. E.
Right arrow Articles by Dayton, W. R.

A Statistically Standardized Muscle Cell Culture Bioassay Measuring the Effect of Swine Serum on Muscle Cell Proliferation1

C. E. Kotts2, M. E. White3, C. E. Allen3, F. Martin4 and W. R. Dayton5

University of Minnesota, St. Paul 55108

Abstract

We have developed a statistically standardized bioassay for quantifying the effect of swine serum on the proliferation rate of cultured L6 myogenic cells. The intra-assay coefficient of variation for this assay is 2.5%. Over 29 experiments, the relationship between the assays response to 2.5% control swine serum and its response to 2.5% serum plus 107 M insulin is linear (r2 = .9). This relationship can be used to adjust experimental data obtained in different experiment to a common control value so that valid inter-assay comparisons can be made. The inter-assay coefficient of variation is reduced four- to five fold by this adjustment procedure. We believe this standardized assay provides a useful system for identifying and isolating unknown growth factors that affect muscle growth rate in an economically important species.

Footnotes

1 Published as paper no. 15160 of the scientific journal series of the Minnesota Agr. Exp. Sta. on research conducted under Minnesota Agr. Exp. St. Project No. 0302- 4816-83. This work was supported by Monsanto Co., Minnesota Agr. Exp. Sta. and a grant from the Natl. Pork Producers Council.

2 Monsanto Co., 700 Chesterfield Parkway, Chesterfield, MO 63198.

3 Dept. of Anim. Sci.

4 Dept. of Appl. Statist.

5 To whom all correspondence and requests for reprints should be addressed.






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1987 by the American Society of Animal Science.