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U.S. Department of Agriculture,6, Miles City, MT 59301 and New Mexico State University Las Cruces 88003
Abstract
Four independent digestion trials with four diets were conducted using four Hereford x Angus steers per trial to evaluate the ability of four internal markers to predict in vivo dry matter digestibility. Diets were 1) cubed alfalfa hay; 2) tall fescue (pre-boot stage, harvested .5 h before feeding); 3) tall wheatgrass hay plus .9 kg soybean meal (SBM)/d and 4) prairie hay. Forages were fed once daily at 90% of ad libitum intake. Separation of internal markers was accomplished by 1) 6-d in vitro fermentation followed by acid detergent fiber (ADF) extraction (IVADF); 2) 6-d in vitro fermentation followed by neutral detergent fiber extraction (IVNDF); 3) ADF extraction followed by 3-h digestion with 72% H2SO4 (ADL) and 4) ADF extraction followed by 10-d cellulase incubation (ADFIC). Dry matter digestibility (DMD) was calculated from the feed:feces ratio of each internal marker and compared with in vivo apparent DMD within each diet. When compared within both cubed alfalfa hay and tall wheatgrass/SBM, digestion coefficients from IVADF ratio and IVNDF ratio were similar (P>.10) to in vivo coefficients. However, digestion coefficients from ADL ratio and ADFIC differed (P<.01) from in vivo apparent digestibilities. When compared within tall fescue and prairie hay, digestion coefficients from all internal markers evaluated were different (P<.01) from in vivo digestion coefficients. Fecal marker recoveries explained observed differences between in vivo DMD and DMD from marker ratios. The relationship between in vivo DMD and DMD determined by IVADF and IVNDF ratio was highly variable. Caution should be exercised in using IVADF and IVNDF as internal markers, particularly with immature, freshly harvested or grazed forage. Acid detergent lignin and ADFIC were least acceptable for use as internal markers in all diets evaluated.
1 Publication has been approved by the Director of the Montana Agr. Exp. Sta., Journal Ser. No. J-1781.
2 Present address: Dept. of Anim. Sci. and Ind., Call Hall, Kansas State Univ., Manhattan 66506.
3 Address reprint requests to this author.
4 Fort Keogh Livestock and Range Res. Lab., USDA-ARS, Route 1, Box 2021, Miles City, MT 59301.
5 Dept. of Anim. and Range Sci., Box 3-I, New Mexico State Univ., Las Cruces 88003.
6 Agr. Res. Serv., Fort Keogh Livestock and Range Res. Lab. with Montana Agr. Exp. Sta. cooperating.
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