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Evaluation of Prostaglandin E₂ as a Regulator of Lipolysis in Bovine Adipose Tissue¹

Iowa State University, Ames 50011

Abstract

Effects of exogenous prostaglandin E₂ (PGE₂) on rates of lipolysis in sections of subcutaneous adipose tissue biopsied from fed and fasted Holstein steers were determined. The interaction of PGE₂ with several exogenous effectors of lipolysis and of the adenylate cyclase-cAMP system also was measured. Epinephrine increased basal (nonstimulated) lipolysis approximately one-fold. Prostaglandin E₂ had no effect on either basal or epinephrine-stimulated lipolysis. Dibutyryl cAMP increased rate of lipolysis .4-fold, whereas theophylline increased lipolysis more than one-fold. Theophylline had an additive effect on epinephrine-stimulated lipolysis. Dibutyryl cAMP increased theophylline-stimulated lipolysis but not epinephrine-stimulated lipolysis. Prostaglandin E₂ had no effect on epinephrine-, dibutyryl cAMP- or theophylline-stimulated lipolysis. Fasting decreased basal lipolysis by 40%. Furthermore, lipolysis in tissue incubated with PGE₂, epinephrine or PGE₂ plus epinephrine decreased from 30 to 50% upon fasting. As also shown with tissue from fed steers, PGE₂ did not alter basal or epinephrine-stimulated lipolysis in tissue from fasted steers. Influences of exogenous effectors on lipolysis in adipose tissue from fed and fasted steers indicate that PGE₂ does not control the adenylate cyclase-cAMP system that regulates lipolysis in bovine adipose tissue.

¹ Journal Paper No. J-11833 of the Iowa Agr. and Home Econ. Exp. Sta., Ames. Project 2187. This research was supported partly by Biomed. Sci. Support Grant No. 5-SO7-RR07034. The authors acknowledge the assistance of Paul Fullerton and Leland Nuehring in performing experimental analyses.

² Present address: Dept. of Nutr. and Food Sci., Texas Woman's Univ., Denton, TX 76204.

³ Present address: Dept. of Sci., Atlantic Christian College, Wilson, NC 27893.

⁴ Dept. of Anim. Sci. and Biochem-Biophys. To whom reprint requests should be addressed.