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Washington State University6, Pullman 99164-6310
Abstract
Eighteen mature horses were used to study protein requirements of working horses. Treatments included intense exercise, medium exercise and maintenance in a 3 x 3 factorial arrangement with three levels of calculated dietary crude protein (CP; 8.5, 7.0 and 5.5%). The horses were on the various exercise-protein treatments for 60 d, then fasted 4 d to evaluate their N status after the treatment period. Exercise had no significant effect on body weight over the feeding and fasting periods. No one exercise or protein treatment expressed the classical low plasma albumin or total protein concentrations of protein-deficient or malnourished animals. Plasma urea N (PUN) concentrations reflected the amount of protein in the diet, with the horses fed the high-protein treatment having the highest PUN concentration (P<.05). Fasting brought about a significant rise in the urinary percentage of urea + NH3 N, with the highest protein treatment excreting the highest percentage (P<.05). Because plasma protein concentrations were maintained and labile protein reserves were apparently not depleted, it appears that the lowest protein diet containing 1.9 g digestible protein/W.75 was adequate, regardless of work load.
1 Scientific Paper No. 6595. College of Agr., Agr. Res. Center, Washington State Univ., Pullman 99164-6240. Project No. 0025.
2 Present address: Poul. Sci. Dept., Univ. of Wisconsin, Madison 53706.
3 Present address: Anim. Sci. Dept., Univ. of Minnesota, St. Paul 55108.
4 Present address: Agribasics, P.O. Box 2548, Great Falls, MO 59403.
5 We greatly appreciate the help of Gail Wagner for organizing and recording individual horse exercise.
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